en عمومى General پژوهشي Research <span style="font-size:12px;"><span style="font-family:Tahoma;"><b><span style="color:#2d73b4">Background: </span></b><span style="color:black">Bisphenol A is a widely used chemical in various industries and has cytotoxic and genotoxic effects. Nicorandil is a drug for the treatment of ischemic heart disease with useful antioxidant and tissue-protective properties. In the present study, nicorandil nanoliposomes coated with tocopheryl polyethylene glycol succinate-1000 were developed to enhance its antioxidant properties, and their protective effects were evaluated against bisphenol A-induced cytotoxicity.</span><br> <b><span style="color:#2d73b4">Methods: </span></b><span style="color:black">Nanoliposomes were prepared by the </span>film<b> </b>hydration method, and the characteristics of the particles, loading efficiency, and release profile were assessed. Human umbilical vein endothelial cells (<span style="color:black">HUVEC</span>) were exposed to<span style="color:black"> bisphenol A (450 &micro;M) after 24-hr pretreatment with nicorandil or nicorandil-nanoliposomes (1-100 &micro;M). Cell viability (MTT assay), intracellular reactive oxygen species (ROS) levels, total antioxidant capacity (TAC), and glutathione content were evaluated. </span><br> <b><span style="color:#2d73b4">Results: </span></b><span style="color:black">Nanoliposomes were obtained with proper </span>particle characteristics and a loading efficiency of 76%. The IC₅₀ of <span style="color:black">bisphenol A was calculated as about 450 &micro;M. </span>P<span style="color:black">retreatment of cells with nicorandil and nicorandil-nanoliposomes</span> <span style="color:black">significantly reduced intracellular ROS levels and increased TAC but could not elevate cellular glutathione content. Nicorandil-nanoliposomes led to a greater decrease in ROS and increase in TAC than did nicorandil.</span><br> <b><span style="color:#2d73b4"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b><span style="color:black">The findings indicate the cytoprotective and antioxidant effects of nicorandil nanoliposomes coated with tocopheryl polyethylene glycol succinate against cytotoxicity induced by bisphenol A.</span></span></span><br> <br> &nbsp; Bisphenol A, Cytotoxicity, Endothelial cells, Nanoliposomes, Nicorandil 0 0 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1510-1&slc_lang=en&sid=1 Mahmoud Etebari etebari@pharm.mui.ac.ir 100319475328460019202 100319475328460019202 No Department of Pharmacology and Toxicology, Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Jaleh Varshosaz varshosaz@pharm.mui.ac.ir 100319475328460019203 100319475328460019203 No Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Leila Safaeian leila_safaeian@pharm.mui.ac.ir 100319475328460019204 100319475328460019204 Yes Department of Pharmacology and Toxicology, Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran Hajar Pourshayesteh hajarfz61@gmail.com 100319475328460019205 100319475328460019205 No Department of Pharmacology and Toxicology, Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran