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:: Volume 6, Issue 18 (Autumn 2012) ::
IJT 2012, 6(18): 691-698 Back to browse issues page
Effect of Acute Toxicity of Cadmium in Mice Kidney Cells
Masoomeh Masoomi Karimi *, Moslem Jafari Sani , Ali Zaree Mahmudabadi , Asma Jafari sani , Seyed Reza Khatibi
Department of Immunology, Torbateheydariyeh University of Medical Sciences, Torbateheydariyeh, Iran.
Abstract:   (11329 Views)
Background: Cadmium is one of the most toxic heavy metals in our environment having a very strong ability to accumulate in body organs, especially in kidney. The present study was done to determine the genotoxicity and cytotoxicity in kidneys of rats exposed to cadmium.
Methods: Male rats (n=30), kept in standard conditions were used in this study. The animals were randomly divided into 2 groups (control and treatment). The treatment group was intraperitoneally injected with Cd (300µm/kg) at hours 0, 6, 12, 24, 48. Twenty four hours after the last injection, the rats were sacrificed and their kidneys were obtained. Then oxidative stress markers, malondialdehide (MDA), glutathione (GSH), and superoxide dismutase (SOD), were assayed in homogenized kidney for studying their cytotoxicity. For genotoxicity and DNA damage studies, Comet assay was run on isolated kidney cells. Data analysis was done by t-test and ANOVA using SPSS software version 15.
Results: MDA and GSH concentrations in normal and Cd exposed kidney cells were 287.01±37.30nmol/g.pr and 15.61±3.89µmol/g.pr and 609.24±87.87nmol/g.pr and 28.52±5.22µmol/g.pr, respectively. In addition, SOD activity in normal and Cd exposed kidney cells were 77.75±4.12 and 218.91±5.40 U/mg.pr, respectively. Comet assay results (content comet length, tail length, and head diameter) showed DNA breakage in the treatment group that was stimulated by Cd which was not seen in the control group.
Conclusion: The results demonstrated the genotoxicity effect of Cd on kidney cells as well as the ability of Cd to producing cytotoxicity.
Keywords: Cadmium, Comet Assay, DNA Damage, Kidney, Oxidative Stress.
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Type of Study: Research | Subject: Special
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Masoomi Karimi M, Jafari Sani M, Zaree Mahmudabadi A, Jafari sani A, Khatibi S R. Effect of Acute Toxicity of Cadmium in Mice Kidney Cells. IJT. 2012; 6 (18) :691-698
URL: http://ijt.arakmu.ac.ir/article-1-148-en.html
Volume 6, Issue 18 (Autumn 2012) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
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