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:: Volume 6, Number 16 (Spring 2012) ::
IJT 2012, 6(16): 615-625 Back to browse issues page
Liver and Kidney Functional Indices of Pregnant Rats Following the Administration of the Crude Alkaloids from Senna alata (Linn. Roxb) Leaves
Musa Toyin Yakubu *, Musa Isah Fakai Musa
Phytomedicine, Toxicology, and Reproductive Biochemistry Research Laboratory, Department of Biochemistry, University of Ilorin, Ilorin, Nigeria. , tomuyak@yahoo.com
Abstract:   (12083 Views)
ABSTRACT
Background:
Alkaloids from Senna alata leaves implicated as the active constituents of abortifacient are yet to be investigated for their effects on the normal functioning of the maternal liver and kidney. Therefore, the effects of crude alkaloids on some biochemical indices of kidney and liver damage were investigated in pregnant rats.
Methods: Pregnant rats were randomized into 4 groups: A (control), B, C, and D and were orally administered 0.5 ml of distilled water, 250, 500 and 1000 mg/kg body weight of the alkaloids respectively once daily on days 10-18 post coitum.
Results: Thin-layer chromatographic separation gave five spots with Rf values of 0.28, 0.33, 0.39, 0.47, and 0.55 that produced creamy precipitate and reddish-brown colour, respectively, with Mayer’s and Wagner’s reagents. Quantitative determination gave 0.30 g which corresponded to a percentage yield of 1.50 % of the alkaloids. The decreases in the activities of alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), aspartate (AST) and alanine transaminases in the liver and kidney of the animals by the alkaloids were accompanied by corresponding increases in the serum enzymes. The alkaloids reduced liver- and kidney-body weight ratios, serum globulin, urea, uric acid, and phosphate ions while the serum concentrations of albumin, bilirubin, creatinine, potassium ions, AST/ALT ratio, blood urea nitrogen: creatinine increased. The levels of sodium, calcium, and chloride ions did not change significantly (P>0.05).
Conclusion: Overall, the alkaloid at doses of 250-1000 mg/kg body weight produced permeability changes in the plasma membrane of the organs and adversely affected the normal secretory, synthetic, and excretory functions of these organs.
Keywords: Alkaloids, function indices, leguminosae, pregnant rats, safety, Senna alata.
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Type of Study: Research | Subject: General
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Yakubu M T, Isah Fakai Musa M. Liver and Kidney Functional Indices of Pregnant Rats Following the Administration of the Crude Alkaloids from Senna alata (Linn. Roxb) Leaves. IJT. 2012; 6 (16) :615-625
URL: http://ijt.arakmu.ac.ir/article-1-123-en.html
Volume 6, Number 16 (Spring 2012) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
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