Volume 16, Issue 2 (May 2022)
IJT 2022, 16(2): 83-90 |
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Mahmoud Ashry1 
, Doaa Galal ELSahra2 , Khaled G. Abdel-Wahhab3 , Mahenor E. Abdelsalam4 , Hagar H. Mourad3 , Alaa M.H. El-Bitar1 , Heba F. Gomaa * 5
, Doaa Galal ELSahra2 , Khaled G. Abdel-Wahhab3 , Mahenor E. Abdelsalam4 , Hagar H. Mourad3 , Alaa M.H. El-Bitar1 , Heba F. Gomaa * 5
1- Zoology Department, Faculty of Science, Al-Azhar University, Assuit, Egypt.
2- Medical Surgical Nursing Department, Faculty of Nursing, Modern Technology and Information University, Cairo, Egypt.
3- Medical Physiology Department, National Research Centre, Giza, Egypt.
4- Department of Biology, Center of Basic Sciences, Misr University for Science & Technology, Giza, Egypt.
5- Department of Zoology, Faculty of Science, Ain-shams University, Cairo, Egypt. ,Shery-yahia2009@hotmail.com
2- Medical Surgical Nursing Department, Faculty of Nursing, Modern Technology and Information University, Cairo, Egypt.
3- Medical Physiology Department, National Research Centre, Giza, Egypt.
4- Department of Biology, Center of Basic Sciences, Misr University for Science & Technology, Giza, Egypt.
5- Department of Zoology, Faculty of Science, Ain-shams University, Cairo, Egypt. ,
Abstract: (1968 Views)
Background: Testicular dysfunction is one of the common side effects that results from the treatment with oxaliplatin® as a chemotherapy drug, and pharmaceutical search for agents to relieve the side effects are underway. The current study explored the possible ameliorative and regenerative effects of Costus ethanolic extract against testicular degeneration in male rats induced by oxaliplatin.
Methods: Male Wistar albino rats weighing 150-200g were divided into four groups of 10 rats each as follows: group-1 control rats; group-2 rats treated orally with the extract at 50 mg/kg/day for six weeks; group-3 rats injected oxaliplatin intraperitoneally at 10 mg/kg/week for six successive weeks; group-4 rats were injected intraperitoneally with oxaliplatin at 10 mg/kg/week combined with a daily oral dose of the Costus extract for six weeks.
Results: Data from the current study revealed that the extract lowered the toxic effect of oxaliplatin on the testicular tissue samples. This was evident by the significant rise in the serum of total & free testosterone and CD4 cells, and the levels of GSH, SOD and CAT activities in the testis coupled with a marked reduction of serum TNF-α and IL-1β and testis MDA, nitric oxide levels and DNA fragmentation. Also, the extract promoted the regeneration of the histopathological structure of the testis.
Conclusion: This study proposes a novel therapeutic application for the Costus extract as a therapeutic agent against testicular toxicity induced by oxaliplatin treatment through its promising anti-inflammatory and antioxidant properties.
Methods: Male Wistar albino rats weighing 150-200g were divided into four groups of 10 rats each as follows: group-1 control rats; group-2 rats treated orally with the extract at 50 mg/kg/day for six weeks; group-3 rats injected oxaliplatin intraperitoneally at 10 mg/kg/week for six successive weeks; group-4 rats were injected intraperitoneally with oxaliplatin at 10 mg/kg/week combined with a daily oral dose of the Costus extract for six weeks.
Results: Data from the current study revealed that the extract lowered the toxic effect of oxaliplatin on the testicular tissue samples. This was evident by the significant rise in the serum of total & free testosterone and CD4 cells, and the levels of GSH, SOD and CAT activities in the testis coupled with a marked reduction of serum TNF-α and IL-1β and testis MDA, nitric oxide levels and DNA fragmentation. Also, the extract promoted the regeneration of the histopathological structure of the testis.
Conclusion: This study proposes a novel therapeutic application for the Costus extract as a therapeutic agent against testicular toxicity induced by oxaliplatin treatment through its promising anti-inflammatory and antioxidant properties.
Keywords: Testicular dysfunction, Oxaliplatin®, Costus, DNA fragmentation, inflammation, Oxidative stress
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