Volume 9, Issue 30 (Autumn 2015)
IJT 2015, 9(30): 1392-1400 |
Back to browse issues page
Musa Toyin Yakubu * 
1, Olalekan Bukunmi Ogunro Oluwayemisi Beatrice Ojewuyi2 , Oluwayemisi Beatrice Ojewuyi3
1, Olalekan Bukunmi Ogunro Oluwayemisi Beatrice Ojewuyi2 , Oluwayemisi Beatrice Ojewuyi3
1- Department of Biochemistry, University of Ilorin, P.M.B 1515, Ilorin, Nigeria. , tomuyak@yahoo.com
2- Department of Biochemistry, University of Ilorin, P.M.B 1515, Ilorin, Nigeria.
3- Department of Biological Sciences, College of Natural Sciences, Al-Hikmah University, Ilorin, Nigeria.
2- Department of Biochemistry, University of Ilorin, P.M.B 1515, Ilorin, Nigeria.
3- Department of Biological Sciences, College of Natural Sciences, Al-Hikmah University, Ilorin, Nigeria.
Abstract: (4432 Views)
Background: The effects of aqueous extract of Fadogia agrestis stem at the doses of 18, 36, and 72 mg/kg body weight on alloxan-induced toxicity was investigated in Wistar rats.
Methods: In total, 35 rats of both sexes (132.80±7.22g) were randomized into five groups (A-E): animals in group A received 0.5 ml of distilled water orally on daily basis for 15 days while the alloxanized rats in groups B, C, D and E also received orally 0.5 ml of distilled water and same volume of the extract corresponding to 18, 36, and 72 mg/kg body weight, respectively after which levels of some biomolecules were determined and histological changes evaluated.
Results: Administration of alloxan significantly (P<0.05) increased blood urea nitrogen (BUN)/creatinine ratio, levels of serum total and conjugated bilirubin, uric acid, Na+, K+, Ca2+ and Cl- as well as activities of alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in the serum whereas activities of ALP, AST and ALT in the liver and kidney, erythrocytes and leucocytes indices, total protein, globulin and PO42- levels as well as albumin/globulin ratio decreased (P<0.05) significantly. Alloxan also caused severe necrosis of the tubular epithelial cells in the nephrons and degeneration of the hepato-cytes. Although all the doses of the plant extract significantly mitigated these changes, the re-versals at 72 mg/kg body weight of the extract compared well (P>0.05) with their respective non-alloxanized distilled water treated control animals in 78% of the parameters investigated.
Conclusion: Overall, the aqueous extract of F. agrestis stem attenuated the alloxan treatment related biochemical, haematological and histological changes in the rats with the 72 mg/kg body weight achieving total reversal in 18 out of the 23 parameters investigated.
Methods: In total, 35 rats of both sexes (132.80±7.22g) were randomized into five groups (A-E): animals in group A received 0.5 ml of distilled water orally on daily basis for 15 days while the alloxanized rats in groups B, C, D and E also received orally 0.5 ml of distilled water and same volume of the extract corresponding to 18, 36, and 72 mg/kg body weight, respectively after which levels of some biomolecules were determined and histological changes evaluated.
Results: Administration of alloxan significantly (P<0.05) increased blood urea nitrogen (BUN)/creatinine ratio, levels of serum total and conjugated bilirubin, uric acid, Na+, K+, Ca2+ and Cl- as well as activities of alkaline phosphatase (ALP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in the serum whereas activities of ALP, AST and ALT in the liver and kidney, erythrocytes and leucocytes indices, total protein, globulin and PO42- levels as well as albumin/globulin ratio decreased (P<0.05) significantly. Alloxan also caused severe necrosis of the tubular epithelial cells in the nephrons and degeneration of the hepato-cytes. Although all the doses of the plant extract significantly mitigated these changes, the re-versals at 72 mg/kg body weight of the extract compared well (P>0.05) with their respective non-alloxanized distilled water treated control animals in 78% of the parameters investigated.
Conclusion: Overall, the aqueous extract of F. agrestis stem attenuated the alloxan treatment related biochemical, haematological and histological changes in the rats with the 72 mg/kg body weight achieving total reversal in 18 out of the 23 parameters investigated.
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |