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Volume 10, Issue 5 (September-October 2016)
IJT 2016, 10(5): 17-22 |
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Ojo O A, Ojo A B, Ajiboye B, Olayide I, Fadaka A. Helianthus annuus Leaf Ameliorates Postprandial Hyperglycaemia by Inhibiting Carbohydrate Hydrolyzing Enzymes Associated with Type-2 Diabetes . IJT 2016; 10 (5) :17-22
URL: http://ijt.arakmu.ac.ir/article-1-505-en.html
URL: http://ijt.arakmu.ac.ir/article-1-505-en.html
Oluwafemi Adeleke Ojo * 
1, Adebola Busola Ojo2 , Basiru Ajiboye3 , Israel Olayide3 , Adewale Fadaka3
1, Adebola Busola Ojo2 , Basiru Ajiboye3 , Israel Olayide3 , Adewale Fadaka3
1- Department of Biochemistry, Afe Babalola University, Ado-Ekiti, Nigeria. , oluwafemiadeleke08@gmail.com
2- Department of Biochemistry, Ekiti State University, Ado-Ekiti, Nigeria.
3- Department of Biochemistry, Afe Babalola University, Ado-Ekiti, Nigeria.
2- Department of Biochemistry, Ekiti State University, Ado-Ekiti, Nigeria.
3- Department of Biochemistry, Afe Babalola University, Ado-Ekiti, Nigeria.
Abstract: (4940 Views)
Background: Diabetes mellitus is a chronic disease. Decreasing postprandial hyperglycemia by retarding glucose absorption through inhibiting carbohydrates digesting enzymes (α-amylase and α-glucosidase) is one of many approaches used for the management of this disease. This study was aimed at evaluating the normoglycaemic potential of Helianthus annuus leaf.
Methods: The effect of the in vitro inhibitory of different extracts (acetone, ethyl acetate and hexane) of the plant was assessed on the activities of diabetes-related enzymes (α-amylase and α-glucosidase).
Results: The hexane extract of H. annuus leaf displayed the best inhibitory activity against α-amylase and α-glucosidase as indicated by the IC50 values (3.92 ± 0.02 mg mL-1) and (3.29 ± 0.12 mg mL-1), respectively. Lineweaver-Burk plot of inhibition of α-amylase and α-glucosidase by this extract showed that it was competitive and non-competitive mode, respectively.
Conclusion: H. annuus leaf possesses hypoglycaemic potential which may be due to the inhibition of pancreatic α-amylase and intestinal α-glucosidase.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
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