:: Volume 11, Issue 2 (March-April 2017) ::
IJT 2017, 11(2): 45-49 Back to browse issues page
Acute Toxicity of an Organophosphate Insecticide Chlorpyrifos to an Anuran, Rana cyanophlyctis
Ajai Kumar Srivastav *, Shilpi Srivastava , Sunil Kumar Srivastav , Nobuo Suzuki
Department of Zoology, DDU Gorakhpur University, Gorakhpur, India. , ajaiksrivastav@hotmail.com
Abstract:   (648 Views)

Background: Chlorpyrifos is an organophosphate pesticide that elicits broad-spectrum insecticidal activity against a number of important arthropod pests. Determining the insecticides’ toxicity to amphibians can give us a better understanding regarding the role of toxicants in amphibian declines. This information would be beneficial to assess their ecological relevance at environmental concentrations. The present study assessed toxicity of chlorpyrifos to an anuran Rana cyanophlyctis.

Methods: For the determination of LC50 values for chlorpyrifos, four-day static renewal acute toxicity test was used. Five replicates each containing ten frogs were subjected to each concentration of chlorpyrifos (2, 4, 6, 8, 10, 12, 14 and 16 mg/L) for the test. Mortality of the frog at different exposure periods (24, 48, 72 and 96 h) was subjected to Probit analysis with the POLO-PC software (LeOra Software) to calculate the LC50 and 95% confidence level.

Results: The LC50 values of chlorpyrifos for the frog R. cyanophlyctis at 24, 48, 72, and 96 h were 8.252, 7.254, 6.247 and 4.993mg/L, respectively.

Conclusion: Mortality has been noticed in chlorpyrifos treated frogs related to the decline in amphibian population. Therefore, chlorpyrifos should not be used near water reservoirs.

Keywords: Amphibian, Anuran, Chlorpyrifos, LC50, Organophosphate, Toxicity
Full-Text [PDF 532 kb]   (388 Downloads)    
Type of Study: Research | Subject: General

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Volume 11, Issue 2 (March-April 2017) Back to browse issues page