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:: Volume 11, Issue 5 (September-October 2017) ::
IJT 2017, 11(5): 23-30 Back to browse issues page
Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment
Peculiar Nwanyibunwa Okoro * , Sani Ibrahim , Hajiya Mairo Inuwa , Stanley Irobekhian Reuben Okoduwa
Department of Basic Research, National Research Institute for Chemical Technology, Zaria, Nigeria. , peckyclett@sironigeria.com
Abstract:   (556 Views)
Background: The global incidence of snakebite has become a major concern to the community. This study aimed to evaluate the effect of mango seed kernel methanol extract on metalloproteases in Carpet Viper (Echis ocellatus) venom.
Methods: Mango seed kernel methanolic extract was evaluated in vitro for its anti-venom activity and inhibition of metalloproteases of Carpet Viper's (Echis ocellatus) venom. Metalloprotease portion was partially purified from the venom of E. ocellatus with a yield of 71%, a purification fold of 2.63 and a specific activity of 19.00 µmol/min/mg protein.
Results: The enzyme appeared as a band on SDS-PAGE with a molecular weight of 23 kDa. The kinetic properties of the enzyme showed a Km of 0.31 mg mL-1 and a Vmax of 9.09 µmol min-1. When the enzyme was incubated with the extract, kinetic studies revealed a mixed non-competitive pattern of inhibition with Km values of 0.56 and 1.11 mg mL-1 and Vmaxvalues of 6.67 and 4.17-µmol min-1 for 5% and 20% inhibitor concentrations, respectively. An estimated Ki value of 0.168 mg mL-1 was obtained from a secondary plot demonstrating that the extract had a high affinity for the partially purified enzyme; thus, could serve as an effective inhibitor.
Conclusion: Methanol extract of mango seed kernel has a high affinity for the partially purified enzyme, and it might provide an inexpensive and readily available alternative to sheep serum in the management of snakebite envenomation. Therefore, further in vivo studies are necessary to assess its effectiveness and safety.
Keywords: Mangifera, Metalloproteases, Snake Bites, Viperidae, Viper Venoms
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Type of Study: Research | Subject: General
1. Mebs D. Venomous and Poisonous Animals: A Handbook for Biologists, Toxicologists and Toxinologists, Physicians and Pharmacists; CRC Press, Medpharm Scientific Publishers: Stuttgart, Germany. 2002; 238–56.
2. Michael GC, Aliyu I, Grema BA. Viper bite on the neck following a fight. Sudan Med Monit 2015; 10:133-6. [DOI:10.4103/1858-5000.171865]
3. Nasidi A. Snakebite as a Serious Public Health Problem for Nigeria and Africa. Presentation to WHO Consultative Meeting on Rabies and Envenomings: by Director, Special Duties, Federal Ministry of Health, Project–Coordinator EchiTAb Study Group, UK/Nigeria on 10 Jan 2007. 2007.
4. Ademola-Majekodumi FO, Oyediran FO, Abubakar SB. Incidence of snakebite in Kaltunga, Gombe State and the efficacy of anew highly purified monovalent antivenom in treating snake bite patients from January 2009 to December 2010. Bull. Soc. Pathol. Exot 2010; 105(3): 175-8. [DOI:10.1007/s13149-012-0232-2]
5. World Health Organisation. Neglected tropical diseases: snakebite. Retrieved October 2015. http://www.who.int/neglected_diseases/en/
6. Iliyasu G, Halliru ST, Habib ZG, Tiamiyu AB, Dayyab FM, Abubakar SB, etal. Blister and bulla following snake bite in Nigeria: A prospective cohort study. Int J Trop Dis Health 2014; 4:1069-77. [DOI:10.9734/IJTDH/2014/12016]
7. Youngmok K., Lounds-Singleton AJ, Talcott ST. Antioxidant phytochemical and quality changes associated with hot water immersion treatment of mangoes (Mangiferaindica L.). Food Chem 2004; 115: 989-93.
8. Pithayanukul P, Leanpolchareanchai J, Bavovada R. Inhibitory effect of tea polyphenols on local tissue damage induced by snake venoms. Phytotherapy research. 2010;24(S1). [DOI:10.1002/ptr.2903]
9. Nzelibe HC, Ahmed HS, Ndidi US. Effect of Methanol Extract of Mangifera indica Linn. Kernel on Partially Purified Phospholipase A2 (PLA2) from Venom of Naja nigricollis: An in vitro Study. Res J Med Plant 2014;8(5):239-45. [DOI:10.3923/rjmp.2014.239.245]
10. Dey A, De JN. Phytopharmacoly of antophidian botanicals: A review. Int J Pharmacol 2012; 8(2): 62-79. [DOI:10.3923/ijp.2012.62.79]
11. Selim SA, Aziz MHA, Mashait MS, Warrad MF. Antibacterial activities, chemical constitutes and acute toxicity of gyptian Origanummajorana L., Peganumharmala L. and Salvia officinalisL. essential oils. Afr J Pharm Pharmacol 2013; 7(13): 725-35.
12. Barkatullah BB, Ibrar MN. Ali Muhammad, N. Rehmanullah. Antispasmodic potential of leaves, barks and fruits of Zanthoxy lumarmatum DC. Afr J Pharm Pharmacol 2013; 7(13): 685-93.
13. Abdulrazaq GH. Effect of Pre-Medication on Early Adverse Reactions Following Antivenom Use in Snakebite. Drug Safety 2011; 34, s10:1-12.
14. Devi CM, Bai MV, Lal AV, Umashankar PR, Krishnan LK. An improved method for isolation of anti vipor venom antibodies from Chicken egg yolk. J Biochem Biophysical Methods 2002; 51(2): 129-38. [DOI:10.1016/S0165-022X(02)00002-7]
15. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5. [DOI:10.1038/227680a0]
16. Mohsen MS, Askera MG, Mahmouda KE, Mohamed S and Aziz A. Purification and characterization of two thermostable protease fractions from Bacillus megaterium. J Genetic Eng Biotechnol 2013;11, s2: 103–09.
17. Chacko N, Ibrahim M, Shetty P. Shastry CS. Evaluation of Antivenom Activity of Calotropis Gigantea Plant Extract against Vipera Russelli Snake Venom. Int J Pharm Sci Res 2012; 3(7): 2272-9.
18. Antony G. Herbs and Herbal constituents active against Snake Bite. Indian J Expt Biol 2010; 48: 865-78.
19. Gutierrez J, Theakston D, Warrell D. Confronting the neglected problem of snake bite envenoming: the need for a global partnership. PloS Med 2006; 3:6. [DOI:10.1371/journal.pmed.0030150]
20. Panfoli I, Calzia D, Ravera S, Morelli A. Inhibition of haemorrhagic snake venom components: old and new approaches. J Toxins 2010; 2: 417-27. [DOI:10.3390/toxins2040417]
21. Sallau AB, Njoku GC, Olabisi AR, Wurocheke AU, Abdulkadir AA, Isah S, Abubakar MS, Ibrahim S. Effect of Guierasenegalensis leaf extract on some Echiscarinatus venom enzymes. J Med Sc 2005;5, s4:280-3.
22. Ibrahim S, Nok JA, Abubakar MS, Sarkiyayi S, Efficacy of Di-n-octyl Phthalate Anti Venom Isolated from Ceibapentandra leaves Extract in Neutralization of EchisocellatusVenom. Res J Appl Sc Eng Technol 2012; 4, s15:2382-7.
23. Sallau AB, Ibrahim MA, Salihu A, Patrick FU. Characterization of phospholipase A2 (PLA2) from Echisocellatus venom. Afr J Biochem Res 2008: 2, s4:098-101.
24. Gomes MSR, Mendes MM, Oliveira F, Andrade RM, Bernardes CP, Hamaguchi A, et al. A new weakly hemorrhagic metalloproteinase from Bothropsmoojeni snake venom. Toxicon 2009; 53: 24–32. [DOI:10.1016/j.toxicon.2008.10.007]
25. Lingott T, Schleberger C, Gutiérrez JM, Merfort I. High-resolution crystal structure of the snake venom metalloproteinase BaP1 complexed with a peptidomimetic: insight into inhibitor binding. Biochemistry 2009;48:6166-74. [DOI:10.1021/bi9002315]
26. Borkow G, Gutiérrez J, Ovadia M. Isolation and characterization of synergistic hemorrhagins from the venom of the snake Bothropsasper. Toxicon 1993; 31(9):1137-50. [DOI:10.1016/0041-0101(93)90129-7]
27. Alam MI. Inhibition of Toxic Effects of Viper and Cobra Venom by Indian Med. Plants Pharmacol Pharm 2014; 5:828-37. [DOI:10.4236/pp.2014.58093]
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Okoro P N, Ibrahim S, Inuwa H M, Okoduwa S I R. Evaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment. IJT. 2017; 11 (5) :23-30
URL: http://ijt.arakmu.ac.ir/article-1-557-en.html

Volume 11, Issue 5 (September-October 2017) Back to browse issues page
مجله سم شناسی و مسمومیتهای ایران Iranian Journal of Toxicology
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