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Volume 11, Issue 5 (September-October 2017)
IJT 2017, 11(5): 13-21 |
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Nurain I O, Bewaji C O. Effects of Aqueous Bark Extracts of Khaya grandifoliola and Enantia chlorantha on Some Biochemical Parameters in Swiss Mice. IJT 2017; 11 (5) :13-21
URL: http://ijt.arakmu.ac.ir/article-1-580-en.html
URL: http://ijt.arakmu.ac.ir/article-1-580-en.html
1- Department of Biochemistry, School of Basic Medical Sciences, Kwara State University, Malete, Nigeria. , ismaila4u2@yahoo.com
2- Department of Biochemistry, Faculty of life Science, University of Ilorin, Ilorin, Nigeria.
2- Department of Biochemistry, Faculty of life Science, University of Ilorin, Ilorin, Nigeria.
Abstract: (6067 Views)
Background: In this study, the potential side effects of Khaya grandifolola (KG) and Enatia chlorantha (EC) were investigated on liver function and hematological parameters of Swiss albino mice infected with malaria.
Method: This study was carried out in part in the Department of Biochemistry, Kwara State University, Malete, and in part in the Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria, 2016. Aqueous extracts of both KG and EC were screened for the presence of some phytochemicals using gas chromatography-mass spectrometry. Five groups of eight animals each were used. Group A was administered with only distilled water. Group B was administered with 50 mg/kg body weight of artemisinin-based combination therapy (ACT). Groups C, D, and E were treated with 400 mg/kg body weight of KG, EC and KG-EC combination, respectively. After 28 d, the animals were sacrificed for biochemical analysis.
Results: The levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin activities were not significantly different (P˂0.05) in all the extract treated animal groups as compared to ACT. However, there was increase in the concentrations of ATL and total bilirubin when compared with that of controls. There was no significant difference (P˂0.05) among Hb, RBC, PCV, WBC, lymphocytes, and platelets compared with ACT. However, they increased as compared to the control groups.
Conclusion: The aqueous bark extracts of KG and EC either in single or in combined form resulted in hepatotoxicity compared to controls. They also have deleterious effects on hematological parameters of the Swiss mice following administration.
Method: This study was carried out in part in the Department of Biochemistry, Kwara State University, Malete, and in part in the Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria, 2016. Aqueous extracts of both KG and EC were screened for the presence of some phytochemicals using gas chromatography-mass spectrometry. Five groups of eight animals each were used. Group A was administered with only distilled water. Group B was administered with 50 mg/kg body weight of artemisinin-based combination therapy (ACT). Groups C, D, and E were treated with 400 mg/kg body weight of KG, EC and KG-EC combination, respectively. After 28 d, the animals were sacrificed for biochemical analysis.
Results: The levels of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and bilirubin activities were not significantly different (P˂0.05) in all the extract treated animal groups as compared to ACT. However, there was increase in the concentrations of ATL and total bilirubin when compared with that of controls. There was no significant difference (P˂0.05) among Hb, RBC, PCV, WBC, lymphocytes, and platelets compared with ACT. However, they increased as compared to the control groups.
Conclusion: The aqueous bark extracts of KG and EC either in single or in combined form resulted in hepatotoxicity compared to controls. They also have deleterious effects on hematological parameters of the Swiss mice following administration.
Keywords: Enantia Chlorantha, Hematologic Tests, Khaya Grandifoliola, Liver Function Tests, Meliaceae
References
1. Baird JK, Caneta-Miguel E, Masbar S, Bustos DG, Abrenica JA, Layawen AV, et al. Survey of resistance to chloroquine of falciparum and vivax malaria in Palawan, The Philippines. T Roy Soc Trop Med 1996;90(4):413-4. [DOI:10.1016/S0035-9203(96)90528-3]
2. Peters W. Drug resistance in Plasmodium berghei, Vincke and Lips, 1948. I. Chloroquine resistance. Exp Parasitol 1965;17(1):80-9. [DOI:10.1016/0014-4894(65)90012-3]
3. Odugbemi TO, Akinsulire OR, Aibinu IE, Fabeku PO. Medicinal plants useful for malaria therapy in Okeigbo, Ondo State, Southwest Nigeria. Afr J Tradit Complement Altern Med 2007;4(2):191-8.
4. Makinde J, Awe S, Agbedahunsi J. Effect of Khaya grandifoliola extract on Plasmodium berghei in mice. Phytother Res 1988;2(1):30-2. [DOI:10.1002/ptr.2650020104]
5. Etim N, Williams ME, Akpabio U, Offiong E. Haematological parameters and factors affecting their values. Agr Sci 2014;2(1):37-47.
6. Oyawoya B, Ogunkunle H. Biochemical and haematological reference values in normal experimental animal. Masson, New York; 2004.
7. Mikolajew M, Kossakowska M, Lewicka S, Kopec M. [Hematological changes in adjuvant-induced disease in rats. Incorporation of various forms of radioactive iron 59-Fe-citrate, 59-Fe-hemoglobin, 59-Fe--old erythrocytes into red blood cells, the liver and spleen]. Reumatologia 1972;10(4):315-24.
8. Schmucker DL. Liver function and phase I drug metabolism in the elderly. Drugs & Aging 2001;18(11):837-51. [DOI:10.2165/00002512-200118110-00005]
9. Bickii J, Njifutie N, Foyere JA, Basco LK, Ringwald P. In vitro antimalarial activity of limonoids from Khaya grandifoliola CDC (Meliaceae). J Ethnopharmacol 2000;69(1):27-33. [DOI:10.1016/S0378-8741(99)00117-8]
10. Wambebe C, Khamofu H, Momoh J, Ekpeyong M, Audu B, Njoku O, et al. Double-blind, placebo-controlled, randomised cross-over clinical trial of NIPRISAN in patients with Sickle Cell Disorder. Phytomedicine 2001;8(4):252-61. [DOI:10.1078/0944-7113-00040]
11. Ibrahim JA, Ayodele EA, Jegede AI, Kunle YF. Comparative studies on Khaya. A. Juss (Meliaceae) in Nigeria. Afr J Biotech 2006;5(11).
12. Olowokudejo J, Kadiri A, Travih V. An ethnobotanical survey of herbal markets and medicinal plants in Lagos State of Nigeria. EBL 2008;2008(1):116-7.
13. Agbaje E, Onabanjo A. Analgesic and antipyretic actions of Enantia chlorantha extract in some laboratory animals. NJNPM 1998;2(1):24-5. [DOI:10.4314/njnpm.v2i1.11776]
14. Zhang X, Organization WH. General guidelines for methodologies on research and evaluation of traditional medicine. WHO. 2000:1-71.
15. Bessey OA, Lowky O, Brock MJ. A method for the rapid determination of alkaline phosphatase with five cubic millimeters of serum. J Biol Chem 1946;164:321-9.
16. Reitman S, Frankel S. Determination of aspartate amino transferase activity in blood serum and tissues. Am J Clin Pathol 1957;25:56-7. [DOI:10.1093/ajcp/28.1.56]
17. Walters MI, Gerarde H. An ultramicromethod for the determination of conjugated and total bilirubin in serum or plasma. Microchem J 1970;15(2):231-43. [DOI:10.1016/0026-265X(70)90045-7]
18. Stephen U, Abiodun F, Osahon O, Ewaen E. Phytochemical analysis and antibacterial activity of Khaya grandifoliola stem bark. J Biol Sci 2009;9(1):63-7. [DOI:10.3923/jbs.2009.63.67]
19. Rates SMK. Plants as source of drugs. Toxicon 2001;39(5):603-13. [DOI:10.1016/S0041-0101(00)00154-9]
20. Farnsworth NR, Soejarto D. Global importance of medicinal plants. Conserv. Med Plants 1991:25-51. [DOI:10.1017/CBO9780511753312.005]
21. Adebayo J, Malomo S. The effect of co-administration of dihydroartemisinin with vitamin E on the activities of cation ATPases in some rat tissues. Nig J Pure Appl Sci 2002;17:1245-52.
22. Murray RK, Granner DK, Mayes PA, Rodwell VW. Harper's illustrated biochemistry: McGraw-Hill; 2014.
23. Adesokan AA, Akanji MA, Yakubu MT. Antibacterial potentials of aqueous extract of Enantia chlorantha stem bark. Afr J Biotech 2007;6(22).
24. Olson S, Wang MG, Carafoli E, Strehler EE, McBride OW. Localization of two genes encoding plasma membrane Ca2(+)-transporting ATPases to human chromosomes 1q25-32 and 12q21-23. Genomics 1991;9(4):629-41. [DOI:10.1016/0888-7543(91)90356-J]
25. Bumah V, Essien E, Agbedahunsi J, Ekah O. Effects of Khaya grandifoliola (Meliaceae) on some biochemical parameters in rats. J Pharmacol 2005;102(3):446-9. [DOI:10.1016/j.jep.2005.07.014]
26. Adedapo AA, Abatan MO, Olorunsogo OO. Effects of some plants of the spurge family on the haematological and biochemical parameters of rats. Vet Arhiv 2007;77(1):29-38.
27. Maton D, Hopkins J, McLaughlin CW, Johnson S, Warner M, LaHart D, et al. Human Biology and Health. Englewood Cliffs, New Jersey, US. Prentice Hall ISBN 0-13-981176-1 OCLC. 1997;32308337:1993.
28. Weed RI, Reed CF, Berg G. Is hemoglobin an essential structural component of human erythrocyte membranes? J Clin Invest 1963;42(4):581-2. [DOI:10.1172/JCI104747]
29. De Villota ED, Carmona MG, Rubio J, DE Andres SR. Equality of the in vivo and in vitro oxygen-binding capacity of haemoglobin in patients with severe respiratory disease. BJA: British J Anaesthesia 1981;53(12):1325-8. [DOI:10.1093/bja/53.12.1325]
30. Morris CR, Kuypers FA, Larkin S, Vichinsky EP, Styles LA. Patterns of arginine and nitric oxide in patients with sickle cell disease with vaso-occlusive crisis and acute chest syndrome. J Pediat Hematol Onc 2000;22(6):515-20. [DOI:10.1097/00043426-200011000-00009]
31. Agbor A, Odetola A. Hematological studies of Parquetina nigrescens on haemorrhagic anaemic rats. AJMMS 2000;30(1-2):105-9.
32. Watson J, Starman AW, Bilello FP. The significance of the paucity of sickle cells in newborn Negro infants. Am J Med Sci 1948;215(4):419-23. [DOI:10.1097/00000441-194804000-00008]
33. Isaac L, Abah G, Akpan B, Ekaette I, editors. Haematological properties of different breeds and sexes of rabbits. Proceedings of the 18th Annual Conference of Animal Science Association of Nigeria; 2013.
34. Laki K. Our ancient heritage in blood clotting and some of its consequences. Ann. N.Y. Acad. Sci 1972;202(1):297-307. [DOI:10.1111/j.1749-6632.1972.tb16342.x]
35. Machlus KR, Thon JN, Italiano JE. Interpreting the developmental dance of the megakaryocyte: a review of the cellular and molecular processes mediating platelet formation. Brit J Haematol 2014;165(2):227-36. [DOI:10.1111/bjh.12758]
36. Ramaiah SK. A toxicologist guide to the diagnostic interpretation of hepatic biochemical parameters. Food Chem Toxicol 2007;45(9):1551-7. [DOI:10.1016/j.fct.2007.06.007]
37. Rezg R, Mornagui B, El-Fazaa S, Gharbi N. Biochemical evaluation of hepatic damage in subchronic exposure to malathion in rats: effect on superoxide dismutase and catalase activities using native PAGE. C R Biol 2008;331(9):655-62. [DOI:10.1016/j.crvi.2008.06.004]
38. Manfo FPT, Nantia EA, Kuete V. Hepatotoxicity and Hepatoprotective Effects of African Medicinal Plants. Toxicol Surv Afr Med Plants 2014:323-55. [DOI:10.1016/B978-0-12-800018-2.00011-X]
39. Afolayan A, Yakubu M. Effect of Bulbine natalensis Baker stem extract on the functional indices and histology of the liver and kidney of male Wistar rats. J Med Food 2009;12(4):814-20. [DOI:10.1089/jmf.2008.0221]
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
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