:: Volume 11, Issue 5 (September-October 2017) ::
IJT 2017, 11(5): 5-11 Back to browse issues page
The Role of Oxidative Stress in Severity of Obstructive Pulmonary Complications in Sputum of Sulfur Mustard-Injured Patients
Javad Heydari , Mahvash Jafari * , Saeed Khazaie , Hassan Goosheh , Mostafa Ghanei , Ashraf Karbasi
Department of Biochemistry, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran. , m.jafari145@gmail.com
Abstract:   (540 Views)
Background: Sulfur mustard (SM) is a strong bifunctional alkylating agent that causes delayed complications in organs such as lung. Oxidative stress plays a pivotal role in the pathogenesis and progression of many pulmonary diseases. The aim of this study was to investigate the oxidative stress in sputum of SM exposed patients with mild, moderate and severe pulmonary dysfunction and assessing their relationship with pulmonary function.
Methods: In this cross–sectional study, oxidative stress biomarkers in sputum were examined on 26 patients with SM-induced bronchiolitis obliterans (9 mild, 14 moderate and 3 severe) and 12 matched healthy controls referred to Baqiyatallah Hospital, Tehran between October 2015 and April 2016.
Results: Sputum superoxide dismutase, catalase and glutathione S-transferase activities and malondialdehyde level in moderate and severe groups were significantly higher than in the control group (P=0.002, P=0.004, P=0.014 and P=0.009, respectively). Glutathione (GSH) level in moderate (22.29%, P=0.025) and severe (45.07%, P=0.004) groups were significantly lower than the control. A decreased in GSH level in severe (41.7%) groups was observed as compared with the mild group. Pearson analysis revealed strong correlations between disease severity and oxidative stress biomarkers in sputum of patients with moderate and severe injuries.
Conclusions: Oxidative stress is involved in the pathogenesis of patients with moderate and severe pulmonary dysfunction following SM exposure. The presence of enhanced oxidative stress relates to the decline lung function and the progression of the disease. Sputum induction in SM-injured patients can be used to the assessment of the antioxidant status of bronchial secretions.
Keywords: Oxidative Stress, Severity of Disease, Sputum, Sulfur Mustard
Full-Text [PDF 194 kb]   (228 Downloads)    
Type of Study: Research | Subject: Special
References
1. Davoodzadeh H, Rahmani H. Systemic Inflammation in Chemical Lung Veterans with Mustard Gas. Persian J Med Sci 2015;2(2):18-29.
2. Veress LA, Anderson DR, Hendry-Hofer TB, Houin PR, Rioux JS, Garlick RB, et al. Airway tissue plasminogen activator prevents acute mortality due to lethal sulfur mustard inhalation. Toxicol Sci 2014;143(1):178-84. [DOI:10.1093/toxsci/kfu225]
3. Razavi SM, Salamati P, Harandi AA, Ghanei M. Prevention and treatment of respiratory consequences induced by sulfur mustard in Iranian casualties. Int J Prev Med 2013;4(4):383-9.
4. Ghanei M, Shohrati M, Jafari M, Ghaderi S, Alaeddini F, Aslani J. N-Acetylcysteine Improves the Clinical Conditions of Mustard Gas Exposed Patients with Normal Pulmonary Function Test. Basic Clin Pharmacol Toxicol 2008;103(5):428-32. [DOI:10.1111/j.1742-7843.2008.00318.x]
5. Karbasi A, Goosheh H, Aliannejad R, Saber H, Salehi M, Jafari M, et al. Pepsin and bile acid concentrations in sputum of mustard gas exposed patients. Saudi J Gastroenterol 2013;19(3):121-5. [DOI:10.4103/1319-3767.111954]
6. Zeng M, Li Y, Jiang Y, Lu G, Huang X, Guan K. Local and systemic oxidative stress and glucocorticoid receptor levels in chronic obstructive pulmonary disease patients. Can Respir J 2013;20(1):35-41. [DOI:10.1155/2013/985382]
7. Tkaczyk J, Vizek M. Oxidative stress in the lung tissue-sources of reactive oxygen species and antioxidant defence. Prague Med Rep 2007;108(2):105-14.
8. Jafari M, Ghanei M. Evaluation of plasma, erythrocytes, and brochoalveolar lavage fluid antioxidant defense system in sulfur mustard-injured patients. Clin Toxicol (Phila) 2010;48(3):184-92. [DOI:10.3109/15563651003623297]
9. Laskin JD, Black AT, Jan YH, Sinko PJ, Heindel ND, Sunil V, et al. Oxidants and antioxidants in sulfur mustard–induced injury. Ann N Y Acad Sci 2010;1203(1):92-100. [DOI:10.1111/j.1749-6632.2010.05605.x]
10. Li J, Chen L, Wu H, Lu Y, Hu Z, Lu B, et al. The mixture of salvianolic Acids from Salvia miltiorrhiza and total flavonoids from Anemarrhena asphodeloides attenuate sulfur mustard-induced injury. Int J Mol Sci 2015;16(10):24555-73. [DOI:10.3390/ijms161024555]
11. Tavilani H, Nadi E, Karimi J, Goodarzi MT. Oxidative stress in COPD patients, smokers, and non-smokers. Respir Care 2012;57(12):2090-4. [DOI:10.4187/respcare.01809]
12. Jafari M. Dose-and time-dependent effects of sulfur mustard on antioxidant system in liver and brain of rat. Toxicology 2007;231(1):30-9. [DOI:10.1016/j.tox.2006.11.048]
13. Pirzad G, Jafari M, Tavana S, Sadrayee H, Ghavami S, Shajiei A, et al. The role of fas-fasl signaling pathway in induction of apoptosis in patients with sulfur mustard-induced chronic bronchiolitis. J Toxicol 2011;2010:373612.
14. Tahmasbpour E, Reza Emami S, Ghanei M, Panahi Y. Role of oxidative stress in sulfur mustard-induced pulmonary injury and antioxidant protection. Inhal Toxicol 2015;27(13):659-72. [DOI:10.3109/08958378.2015.1092184]
15. Shohrati M, Ghanei M, Shamspour N, Jafari M. Activity and function in lung injuries due to sulphur mustard. Biomarkers 2008;13(7):728-33. [DOI:10.1080/13547500802646622]
16. Shohrati M, Ghanei M, Shamspour N, Babaei F, Abadi MN, Jafari M, et al. Glutathione and malondialdehyde levels in late pulmonary complications of sulfur mustard intoxication. Lung 2010;188(1):77-83. [DOI:10.1007/s00408-009-9178-y]
17. Antus B. Oxidative stress markers in sputum. Oxid Med Cell Longev 2016;2016:2930434. [DOI:10.1155/2016/2930434]
18. Mirsadraee M, Ghobadi-Marallu H, Khakzad MR, M Lari S, Attaran D, Towhidi M, et al. Level of eosinophil cationic protein in sputum of chemical warfare victims. Iran J Basic Med Sci 2011;14(3):249-55.
19. Beeh KM, Beier J, Koppenhoefer N, Buhl R. Increased glutathione disulfide and nitrosothiols in sputum supernatant of patients with stable COPD. Chest 2004;126(4):1116-22. [DOI:10.1378/chest.126.4.1116]
20. Winterbourn CC, Hawkins RE, Brian M, Carrell RW. The estimation of red cell superoxide dismutase activity. J Lab Clin Med 1975;85(2):337-41.
21. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6. [DOI:10.1016/S0076-6879(84)05016-3]
22. Habig W, Jakoby W. Glutathione S-transferases (rat and human). Methods in Enzymol 1981;77:218-31. [DOI:10.1016/S0076-6879(81)77029-0]
23. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal Biochem 1969;27(3):502-22. [DOI:10.1016/0003-2697(69)90064-5]
24. Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chim Acta 1978;90(1):37-43. [DOI:10.1016/0009-8981(78)90081-5]
25. Harju T, Mazur W, Merikallio H, Soini Y, Kinnula VL. Glutathione-S-transferases in lung and sputum specimens, effects of smoking and COPD severity. Respir Res 2008;9(80):1-10. [DOI:10.1186/1465-9921-9-80]
26. To M, Takagi D, Akashi K, Kano I, Haruki K, Barnes PJ, et al. Sputum plasminogen activator inhibitor-1 elevation by oxidative stress-dependent nuclear factor-κB activation in COPD. Chest 2013;144(2):515-21. [DOI:10.1378/chest.12-2381]
27. Mirbagheri L, Habibi Roudkenar M, Imani Fooladi AA, Ghanei M, Nourani MR. Downregulation of super oxide dismutase level in protein might be due to sulfur mustard induced toxicity in lung. Iran J Allergy Asthma Immunol 2013;12(2):153-60.
28. Jafari M, Pirzad G, Zaree A, Saberi M. Study of the effect of sulfur mustard on cells viability and DNA fragmentation in the human skin fibroblast cells. J Mil Med 2014;16(1):45-51.
29. Dauletbaev N, Rickmann J, Viel K, Buhl R, Wagner TO, Bargon J. Glutathione in induced sputum of healthy individuals and patients with asthma. Thorax 2001;56(1):13-8. [DOI:10.1136/thorax.56.1.13]
30. Deveci F, Ilhan N, Turgut T, Akpolat N, Kirkil G, Muz MH. Glutathione and nitrite in induced sputum from patients with stable and acute asthma compared with controls. Ann Allergy Asthma Immunol 2004;93(1):91-7. [DOI:10.1016/S1081-1206(10)61452-4]
31. Moussa SB, Sfaxi I, Tabka Z, Saad HB, Rouatbi S. Oxidative stress and lung function profiles of male smokers free from COPD compared to those with COPD: a case-control study. Libyan J Med 2014;9(1):23873-4. [DOI:10.3402/ljm.v9.23873]
32. Malaviya R, Sunil VR, Cervelli J, Anderson DR, Holmes WW, Conti ML, et al. Inflammatory effects of inhaled sulfur mustard in rat lung. Toxicol Appl Pharmacol 2010;248(2):89-99. [DOI:10.1016/j.taap.2010.07.018]
33. MacNee W. Pulmonary and systemic oxidant/antioxidant imbalance in chronic obstructive pulmonary disease. Proc Am Thorac Soc 2005;2(1):50-60. [DOI:10.1513/pats.200411-056SF]
34. Harju TH, Peltoniemi MJ, Rytila PH, Soini Y, Salmenkivi KM, Board PG, et al. Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients. Respir Res 2007;8(1):48-9. [DOI:10.1186/1465-9921-8-48]
35. Nadeem A, Raj HG, Chhabra SK. Increased oxidative stress and altered levels of antioxidants in chronic obstructive pulmonary disease. Inflammation 2005;29(1):23-32. [DOI:10.1007/s10753-006-8965-3]
36. Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5(1):9-19. [DOI:10.1097/WOX.0b013e3182439613]



XML     Print



Volume 11, Issue 5 (September-October 2017) Back to browse issues page