Volume 14, Issue 2 (May 2020)
IJT 2020, 14(2): 93-104 |
Back to browse issues page
Amos Ndarubu Tsado1 
, Audu Ali Jigam2 , Helmina Olufunmilola Akanya2 , Ifeanyi Famous Ossamulu2 , Stephen Damola Ariyeloye * 3
, Audu Ali Jigam2 , Helmina Olufunmilola Akanya2 , Ifeanyi Famous Ossamulu2 , Stephen Damola Ariyeloye * 3
1- Department of Basic and Applied Sciences, Niger State Polytechnic P.M.B. 01, Zungeru, Nigeria.; Department of Biochemistry, School of Life Sciences, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria.
2- Department of Biochemistry, School of Life Sciences, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria.
3- Department of Biochemistry, Institute of Health Sciences, Anadolu University, Eskisehir, Turkey. , ariyeloye@gmail.com
2- Department of Biochemistry, School of Life Sciences, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria.
3- Department of Biochemistry, Institute of Health Sciences, Anadolu University, Eskisehir, Turkey. , ariyeloye@gmail.com
Abstract: (2621 Views)
Background: Newbouldia laevis and Crateva adansonii are popular plants used traditionally in the management of patients with malaria infection in Nigeria. In this study, the acute toxicity and anti-plasmodial effects of the leaf extracts of Newbouldia laevis and Crateva adansonii were investigated in mice.
Methods: The Anti-plasmodial activities of both extracts were investigated individually and combined in mice infected with the chloroquine sensitive ANKA-65 Plasmodium berghei strain. Five groups of four mice each were used in our experiments. The LD50 was determined, using the line equation of the mortality against dose levels plot.
Results: The extracts of N. laevis and C. adansonii had a safety level of 200 mg/kg (LD50= 471.43 mg/kg) and 600 mg/kg (LD50=3,500 mg/kg), respectively. Each experimental group was infected with P. berghei strain. The percent inhibition of parasitemia induced by the extracts of N. laevis and C. adansonii were 30.14±2.88% and 61.35±1.41%, respectively, compared to the 78.89% achieved for the standard drug (chloroquine). Mice treated with the combined extracts had a parasite inhibition of 24.23±0.86%. Upon the analysis of the extracts, there were tannins, steroids, flavonoids, saponins and alkaloids in both. The quantitative analyses revealed that tannins were the most abundant (261.85±4.76 mg/100 g & 92.71±6.58 mg/100 g) while saponins were the least abundant (15.09±1.13 mg/100 g & 14.08±1.28 mg/100 g) phytochemicals in both extracts.
Conclusion: The findings support the notion that the traditional use of either plant in the management of malaria in Nigeria appears to be logical.
Methods: The Anti-plasmodial activities of both extracts were investigated individually and combined in mice infected with the chloroquine sensitive ANKA-65 Plasmodium berghei strain. Five groups of four mice each were used in our experiments. The LD50 was determined, using the line equation of the mortality against dose levels plot.
Results: The extracts of N. laevis and C. adansonii had a safety level of 200 mg/kg (LD50= 471.43 mg/kg) and 600 mg/kg (LD50=3,500 mg/kg), respectively. Each experimental group was infected with P. berghei strain. The percent inhibition of parasitemia induced by the extracts of N. laevis and C. adansonii were 30.14±2.88% and 61.35±1.41%, respectively, compared to the 78.89% achieved for the standard drug (chloroquine). Mice treated with the combined extracts had a parasite inhibition of 24.23±0.86%. Upon the analysis of the extracts, there were tannins, steroids, flavonoids, saponins and alkaloids in both. The quantitative analyses revealed that tannins were the most abundant (261.85±4.76 mg/100 g & 92.71±6.58 mg/100 g) while saponins were the least abundant (15.09±1.13 mg/100 g & 14.08±1.28 mg/100 g) phytochemicals in both extracts.
Conclusion: The findings support the notion that the traditional use of either plant in the management of malaria in Nigeria appears to be logical.
Keywords: Toxicity, Plasmodium berghei infection, Crateva adansonii, Newbouldia laevis, Leaf extracts
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |