AU - Hassanshahian, Mehdi TI - Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils PT - JOURNAL ARTICLE TA - IJT JN - IJT VO - 10 VI - 4 IP - 4 4099 - http://ijt.arakmu.ac.ir/article-1-487-en.html 4100 - http://ijt.arakmu.ac.ir/article-1-487-en.pdf SO - IJT 4 ABĀ  - Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran. Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degrading strains was performed using PCR based on amplifying of 16s rDNA. Results: The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environment. Three diazinon-degrading bacteria strains belong to: Pseudomonas putida strain D3, P. fluorescens strain D1 and Achromobacter piechaudii strain D8. The best degrading strain (D1), up to 100 ppm, illustrated a good growth, whereas more than this concentration, the growth was reduced. The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria. Conclusion: The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria. Using these strains and other biological reclamation methods we can eliminate bio-environmental problems. CP - IRAN IN - LG - eng PB - IJT PG - 13 PT - Research YR - 2016