en عمومى General پژوهشي Research <span style="font-size:12px;"><span style="font-family:Tahoma;"><b><span style="color:#2d73b4">Background: </span></b>Given the propensity of standard antidiabetic medications to elicit undesirable side effects over extended periods, natural product derived from medicinal plants have emerged as a compelling area of investigation for their potential therapeutic value and reduced toxicity. <span lang="EN">The present study examined <i>Cotoneaster</i> manna extract for its bioactive compounds.</span><span style="font-size:8.0pt"></span><br> <b><span style="color:#2d73b4">Methods: </span></b>The <i>in vitro</i> cytotoxicity of the extract was assessed on HT29 colon cancer cells and HEK293 normal cells using the MTT assay. The antidiabetic potential of the extract was investigated <i>in vivo</i> using a streptozotocin-induced diabetic rat model. The <i>in vivo</i> antioxidant efficacy was investigated using a D-galactose-induced rat model of aging.<span lang="EN" style="font-size:8.0pt"></span><br> <b><span style="color:#2d73b4">Results: </span></b><span lang="EN">The extract reduced HT29 cell viability in a concentration-dependent manner (<i>p</i><0.05), while exhibiting lower toxicity toward HEK293 cells.</span> <span lang="EN">In diabetic rats, liver histopathology demonstrated partial restoration of tissue architecture and reduced hepatocellular necrosis compared with untreated controls. </span>Compared to the D-galactose group, the extract significantly (<i>p</i><0.05) elevated catalase and superoxide dismutase activities and suppressed malondialdehyde formation in both serum and liver tissues.<br> <b><span style="color:#2d73b4"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b><i><span lang="EN">Cotoneaster</span></i><span lang="EN"> manna exhibits hypoglycemic, anticancer, and antioxidant properties, likely attributable to its phenolic and flavonoid constituents. Further molecular and histopathological studies are warranted to confirm these mechanisms.</span></span></span><br> &nbsp; Anticancer, Antioxidant, Cotoneaster manna, Diabetes, Histopathology 0 0 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1479-1&slc_lang=en&sid=1 Hakimeh Lotfian h.k.lotfian2@gmail.com 100319475328460019343 100319475328460019343 No Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran. Zahra Setayesh-Mehr setayeshmehr@uoz.ac.ir 100319475328460019344 100319475328460019344 Yes Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran. Mohammad Reza Hajinezhad hajinezhad@gmail.com 100319475328460019345 100319475328460019345 No Department of Basic Veterinary Science, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran. Sedigheh Esmailzadeh Bahabadi shirin_esm@yahoo.com 100319475328460019346 100319475328460019346 No Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran.