Iranian Journal of Toxicology

en The Hepato-Cardioprotective Effect of Thiamine in a CCl4-Induced Oxidative Stress Rat Model by Enhancing Hepatic and Cardiac Antioxidant Potential تخصصي Special پژوهشي Research Background: Thiamine deficiency heightens tissue sensitivity to toxins by increasing oxidative stress and promoting inflammation. Various toxins deplete glutathione (GSH) levels, which are essential for cellular signaling, particularly within the NF-κB pathway that regulates responses to harmful substances. Maintaining balance in the NF-κB pathway and GSH is vital for toxin protection. We examined rat liver and heart tissues exposed to carbon tetrachloride (CCl4). Methods: A total of 40 rats were allocated into four experimental groups: group 1 (control), group 2 (control supplemented with thiamine), group 3 (OSR-untreated), and group 4 (OSR supplemented with thiamine). The rats in the thiamine-treated groups were given 180 mg/L daily for a period of two weeks through their drinking water. Oxidative stress was induced by injecting 1 ml/kg of CCl4 intraperitoneally. Levels of GSH, GSSG, and NF-κB expression were assessed, along with indicators of liver and heart function. Liver tissue was analyzed under a microscope by a pathologist. Results: Thiamine improved hepatic and cardiac function, reduced inflammation, and enhanced the antioxidant capacity of the body, liver, and heart against oxidative stress. Additionally, it downregulated NF-κB activity in both the liver and heart and prevented hepatic damage and fat accumulation, with effects reaching statistical significance (p<0.001). Conclusion: Thiamine protected the liver and heart against oxidative stress-induced damage by  enhancing antioxidant capacity and reducing NF-κB signaling and inflammation. This protection was achieved through  boosting the body's ability to fight oxidative stress and lowering biomolecule oxidation. Downregulation of NF-κB levels and enhancement of GSH metabolism appears to be crucial ways in which thiamine safeguards both the liver and the heart.   Heart, Liver, Nuclear factor- kβ, Oxidative stress, Reduced glutathione, Thiamine 0 0 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1513-1&slc_lang=en&sid=1 Sana Irani sanair27@gmail.com 100319475328460019374 100319475328460019374 No Department of Clinical Biochemistry, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran. Sina Mahdavifard s.mahdavifard@qums.ac.ir 100319475328460019373 100319475328460019373 Yes Department of Clinical Biochemistry, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.