Iranian Journal of Toxicology
مجله سم شناسی و مسمومیتهای ایران
IJT
Medical Sciences
http://ijt.arakmu.ac.ir
1
admin
2008-2967
2251-9459
8
10.61186/ijt
14
8888
13
en
jalali
1388
6
1
gregorian
2009
9
1
2
3
online
1
fulltext
en
Genotoxic and Antigenotoxic Activity of Silymarin by an In Vivo Bone Marrow Micronucleus Assay and Comet Assay
Genotoxic and Antigenotoxic Activity of Silymarin by an In Vivo Bone Marrow Micronucleus Assay and Comet Assay
عمومى
General
پژوهشي
Research
<span style="font-weight: bold">Introduction:</span> Silymarin is obtained from Silybum marianum (milk thistle), an edible plant that has long been used medicinally for the treatment of liver-related disorders. Silymarin is a powerful hepatoprotective and antioxidant but the anticlastogenic activity, which is an important aspect of its cancer chemoprevention is not known hence the present investigation was carried out to study its anticlastogenic and DNA protective activity in Balb/c Mouse.<br><span style="font-weight: bold">Material & Methods:</span> After silymarin injections, the mice were administered either cyclophosphamide (CP) or mitomycin C (MMC) intraperitoneally and were sacrificed at 24 hrs, 48 hrs and 72 hrs after the last dose. In vivo bone marrow micronucleus assay and Comet assay in whole blood were performed. <br><span style="font-weight: bold">Results: </span>Silymarin produced an increased number of micronucleated polychromatic erythrocytes in in vivo bone marrow micronucleus assay. Similarly it showed anticlastogenic activity against mutagens cyclophosphamide (CP) and mitomycin C (MMC) and caused significant reduction in the number of micronucleated polychromatic erythrocytes. In comet assay, it produced DNA damage, however silymarin showed DNA protective action against mutagens CP and MMC. <br><span style="font-weight: bold">Conclusion:</span> Silymarin has chemopreventive potentials against CP and MMC induced clastogenicity in Balb/c mice. Ironically, it has also been found to induce clastogenicity and DNA damaging effects. Hence, large-scale investigations are essential to confirm the clastogenic effect before continuing the use of silymarin for therapeutic purposes.
Silymarin, Clastogens, Micronucleus Tests , Bone Marrow, Comet Assay
Silymarin, Clastogens, Micronucleus Tests , Bone Marrow, Comet Assay
203
210
http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-2-44&slc_lang=en&sid=1
S
Kaleeswaran
S
Kaleeswaran
drkalees23@gmail.com
10031947532846003331
10031947532846003331
Yes
Department of Veterinary Pharmacology, Madras Veterinary College, Vepery, Chennai. India
Department of Veterinary Pharmacology, Madras Veterinary College, Vepery, Chennai. India
P
Sriram
P
Sriram
10031947532846003332
10031947532846003332
No
Department of Veterinary Pharmacology, Madras Veterinary College, Vepery, Chennai. India
Department of Veterinary Pharmacology, Madras Veterinary College, Vepery, Chennai. India
C
Vijay Kumar
C
Vijay Kumar
10031947532846003333
10031947532846003333
No
Department of Veterinary Pharmacology, Madras Veterinary College, Vepery, Chennai. India
Department of Veterinary Pharmacology, Madras Veterinary College, Vepery, Chennai. India
L.N
Mathuram
L.N
Mathuram
10031947532846003334
10031947532846003334
No
Department of Veterinary Pharmacology, Madras Veterinary College, Vepery, Chennai. India
Department of Veterinary Pharmacology, Madras Veterinary College, Vepery, Chennai. India