Iranian Journal of Toxicology
مجله سم شناسی و مسمومیتهای ایران
IJT
Medical Sciences
http://ijt.arakmu.ac.ir
1
admin
2008-2967
2251-9459
8
10.61186/ijt
14
8888
13
en
jalali
1399
2
1
gregorian
2020
5
1
14
2
online
1
fulltext
en
Acute Toxicity Studies and Anti-plasmodial Potentials of Newbouldia laevis and Crateva adansonii in Plasmodium Berghei-infected Mice
عمومى
General
پژوهشي
Research
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<div style="text-align: justify;"><strong>Background</strong>: Newbouldia laevis and Crateva adansonii are popular plants used traditionally in the management of patients with malaria infection in Nigeria. In this study, the acute toxicity and anti-plasmodial effects of the leaf extracts of Newbouldia laevis and Crateva adansonii were investigated in mice.<br>
<strong>Methods</strong>: The Anti-plasmodial activities of both extracts were investigated individually and combined in mice infected with the chloroquine sensitive ANKA-65 Plasmodium berghei strain. Five groups of four mice each were used in our experiments. The LD50 was determined, using the line equation of the mortality against dose levels plot. <br>
<strong>Results</strong>: The extracts of N. laevis and C. adansonii had a safety level of 200 mg/kg (LD50= 471.43 mg/kg) and 600 mg/kg (LD50=3,500 mg/kg), respectively. Each experimental group was infected with P. berghei strain. The percent inhibition of parasitemia induced by the extracts of N. laevis and C. adansonii were 30.14±2.88% and 61.35±1.41%, respectively, compared to the 78.89% achieved for the standard drug (chloroquine). Mice treated with the combined extracts had a parasite inhibition of 24.23±0.86%. Upon the analysis of the extracts, there were tannins, steroids, flavonoids, saponins and alkaloids in both. The quantitative analyses revealed that tannins were the most abundant (261.85±4.76 mg/100 g & 92.71±6.58 mg/100 g) while saponins were the least abundant (15.09±1.13 mg/100 g & 14.08±1.28 mg/100 g) phytochemicals in both extracts. <br>
<strong>Conclusion</strong>: The findings support the notion that the traditional use of either plant in the management of malaria in Nigeria appears to be logical.<br>
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<address dir="rtl" style="margin-right: 80px;"></address>
Toxicity, Phytochemicals, Malaria, Plasmodium berghei, Crateva adansonii, Newbouldia laevis.
Toxicity, Plasmodium berghei infection, Crateva adansonii, Newbouldia laevis, Leaf extracts
93
104
http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-636-1&slc_lang=en&sid=1
Amos Ndarubu
Tsado
infoamosandarubu@gmail.com
10031947532846009174
10031947532846009174
No
Department of Basic and Applied Sciences, Niger State Polytechnic P.M.B. 01, Zungeru, Nigeria.; Department of Biochemistry, School of Life Sciences, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria.
Audu Ali
Jigam
aajigam@gmail.com
10031947532846009175
10031947532846009175
No
Department of Biochemistry, School of Life Sciences, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria.
Helmina Olufunmilola
Akanya
funmiakanya@yahoo.com
10031947532846009176
10031947532846009176
No
Department of Biochemistry, School of Life Sciences, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria.
Ifeanyi Famous
Ossamulu
ossafame@gmail.com
10031947532846009177
10031947532846009177
No
Department of Biochemistry, School of Life Sciences, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria.
Stephen Damola
Ariyeloye
ariyeloye@gmail.com
10031947532846009178
10031947532846009178
Yes
Department of Biochemistry, Institute of Health Sciences, Anadolu University, Eskisehir, Turkey.