Iranian Journal of Toxicology
مجله سم شناسی و مسمومیتهای ایران
IJT
Medical Sciences
http://ijt.arakmu.ac.ir
1
admin
2008-2967
2251-9459
8
10.61186/ijt
14
8888
13
en
jalali
1386
12
1
gregorian
2008
3
1
2
1
online
1
fulltext
en
Depletion of Ovarian Primordial Follicles after Gamma Irradiation
Depletion of Ovarian Primordial Follicles after Gamma Irradiation
عمومى
General
پژوهشي
Research
<span style="font-weight: bold">Introduction:</span>Artifical sourace of radiation have increased worldwide average annual exposure of mankind and could especially affect rapidly dividing cells in gonads. Since, radiation sensitivities of different components of primordial follicles, and the time required for their deplation have not been morphologically eatablished we decided to estimate the time required for depletion of primordial follicles and to analyze the morphological changes in primordial follicles after gamma irradiation in rats ovaries.
<br><span style="font-weight: bold">Material & Method: </span>108-Female albino rats of 3 weeks age were divided into three groups. Out of them, 36 animals received 4.5 Gray, and 36 received 8.3 Gray of gamma radiation while rest received sham radiation. Then their Ovaries were dissected at 2hrs, 4hrs, 6hrs, one day, 7 days and 14 days after irradiation. Histological examination of five random sections of each ovary was performed.
<br><span style="font-weight: bold">Results:</span> The stockpile of primordial follicles almost depleted within 24 hours. The pool of primordial follicles was greatly suppressed after irradiation showing primordial follicles radiosensitivity. Intense necrosis was evident with karyorrhexis and karyolysis in nucleus of oocyte while effects on granulosa cells were little and they increased the bulk of interstitial tissues.
<br><span style="font-weight: bold">Conclusion:</span>Gamma ionizing radiation induces intense and rapid necrotic degeneration of oocyte in primordial ovarian follicles. The granulosa cells of degenerated primordial follicle increase the bulk of ovarian interstitial tissue. Resultant ovaries do not have enough follicular reserve for ovulatory process which may lead to early cessation of menstruation and eventually infertility.
Gamma radiation Ovary, Oocyte, Granulosa Cells, Necrosis, Rats
Gamma radiation Ovary, Oocyte, Granulosa Cells, Necrosis, Rats
6
6
http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-2-56&slc_lang=en&sid=1
N
Tawakal
Tawakal
10031947532846003382
10031947532846003382
No
Department of Anatomy, King Edward Medical University, Lahore Pakistan
Department of Anatomy, King Edward Medical University, Lahore Pakistan
A
Mubarak Khalid
Mubarak Khalid
10031947532846003383
10031947532846003383
No
Department of Anatomy, King Edward Medical University, Lahore Pakistan
Department of Anatomy, King Edward Medical University, Lahore Pakistan
U
Farooq Khan
Farooq Khan
10031947532846003384
10031947532846003384
No
Lahore General Hospital, Lahor Pakistan
Lahore General Hospital, Lahor Pakistan
K
Raees Ahmad
Raees Ahmad
10031947532846003385
10031947532846003385
No
Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan
Department of Biological Sciences, University of Sargodha, Sargodha, Pakistan
Asmatullsh
Asmatullsh
dr.asmatullah@gmail.com
10031947532846003386
10031947532846003386
Yes
Department Zoology, University of the Punjab, Lahor, Pakistan
Department Zoology, University of the Punjab, Lahor, Pakistan