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Volume 11, Issue 1 (January-Fabruary 2017)                   IJT 2017, 11(1): 27-32 | Back to browse issues page

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Koohi M K, Staji H, Hayati F, Ghazi-Khansari M, Jamshidi R, Shahroozian E. Co-Exposure Effects of LPS with Various Aflatoxin B1 Doses in Isolated Perfused Rat Liver Model. IJT 2017; 11 (1) :27-32
URL: http://ijt.arakmu.ac.ir/article-1-530-en.html
Co-Exposure Effects of LPS with Various Aflatoxin B1 Doses in Isolated Perfused Rat Liver Model
Mohammad Kazem Koohi1 , Hamid Staji2 , Farzad Hayati3 , Mahmoud Ghazi-Khansari4 , Reza Jamshidi5 , Ebrahim Shahroozian * 6
1- Department of Basic Science, Tehran University, Tehran, Iran.
2- Department of Pathobiology, Semnan University, Semnan, Iran.
3- Department of Clinical sciences, Semnan University, Semnan, Iran.
4- Department of Pharmacology, Tehran University of Medical Science, Tehran, Iran.
5- Department of Basic Science, Semnan University, Semnan, Iran.
6- Department of Basic Science, Semnan University, Semnan, Iran. , shahroozian@semnan.ac.ir
Abstract:   (5403 Views)

Background: Activation of inflammatory cells can cause more chemicals induced-hepatotoxicity. Aflatoxin B1 (AFB1) is a fungal toxin that induces acute hepatotoxicity in humans and animals. This study was conducted to examine the effect of co-exposure LPS and various aflatoxin B1 doses on the damage hepatic parameters in isolated perfused rat liver.

Methods: Thirty-two male wistar rats (250-300g) were divided to eight groups including Control and LPS; three groups with various doses of AFB1 (0.01, 0.1 and 1 ppm) and three groups with various doses of AFB1 and Lipopolysaccharide (LPS) (300 ppm). Activity of Aspartate transaminase (AST) and Alanine transaminase (ALT) were determined in perfusate. Thiobarbituric acid reactive substances (TBARS) and Glutathione (GSH) concentrations were measured in homogenate liver.

Results: At two groups of AFB 1 (with LPS and without LPS) at AFB1 concentrations of 0.1 and 1 ppm, elevation of AST and ALT enzymes activity were indicated. Values of GSH in both of groups (AFB 1 with LPS and without LPS) had reduced at concentration of 1 ppm. TBARS concentrations were enhanced in AFB1 concentration of 1 ppm in both of groups (with LPS and without LPS), however in comparison between groups (with LPS and without LPS) in similar concentrations significant different did not observe (P<0.05).

Conclusion: Non-injurious dose of LPS did not enhance liver susceptibility to various doses of AFB1 in perfused rat liver. This may be in part of due to extrahepatic factors, which contribute, in more liver damage.

Keywords: Aflatoxin B-1, Isolated Perfused Rat Liver, Lps.
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Type of Study: Research | Subject: General
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