Volume 12, Issue 1 (January-Fabruary 2018)
IJT 2018, 12(1): 19-26 |
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Rasheed Bolaji Ibrahim * 
1, Jubril Olayinka Akolade2 , Abdulhakeem Olarewaju Sulyman3 , Saheed Sabiu3 , Babalola Ola Yusuf4 , Lateefah Badrudeen Yusuf3
1, Jubril Olayinka Akolade2 , Abdulhakeem Olarewaju Sulyman3 , Saheed Sabiu3 , Babalola Ola Yusuf4 , Lateefah Badrudeen Yusuf3
1- Department of Biochemistry, Faculty of Pure and Applied Sciences, Kwara State University, Malete, Ilorin, Nigeria. , ibrahimrasheedb@gmail.com
2- PhD of Biochemistry, Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex, Sheda, Abuja, Nigeria.
3- Department of Biochemistry, Faculty of Pure and Applied Sciences, Kwara State University, Malete, Ilorin, Nigeria.
4- Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria.
2- PhD of Biochemistry, Biotechnology and Genetic Engineering Advanced Laboratory, Sheda Science and Technology Complex, Sheda, Abuja, Nigeria.
3- Department of Biochemistry, Faculty of Pure and Applied Sciences, Kwara State University, Malete, Ilorin, Nigeria.
4- Department of Biochemistry, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria.
Abstract: (5681 Views)
Background: Heliotropium indicum is used by traditional medical practitioners in North Central Nigeria for the management of ailments including diabetes. However, the folkloric use of H. indicum as antidiabetic has been asserted, but its roles on the hyperglycemia-induced organ-specific complications are not yet scientifically proven. Thus, ameliorative effect of aqueous leaf extract of H. indicum on selected toxicological parameters in hyperglycaemic rats was investigated in this study.
Methods: Twenty-five rats were randomized into five groups. The study was carried out at the Animal Holding Unit, Biochemistry Department, University of Ilorin in 2013. Four groups were intraperitoneally administered singly with 150 mg/kg b.wt of alloxan to induce hyperglycemia. The normal control (NC) and hyperglycaemic control (HC) groups were administered 1 ml distilled water, while the reference group (HR) were administered 14.2 mg/kg b.wt of metformin and the test groups, H30 and H75 were administered 30 and 75 mg/kg b.wt, the extract respectively for fourteen days.
Results: The significantly increased (P<0.05) serum concentrations of tissue membrane bound enzymes (ALT, AST, ACP and ALP), direct and total bilirubin, urea and creatinine in HC indicating compromised tissue structures and functions in HC were attenuated. The significantly (P<0.05) reduced serum total protein, globulin and albumin in HC were significantly increased by both doses of the extract. The ameliorative role of the extract at the test doses was supported by the histological assessment of liver and kidney of the animals.
Conclusion: Aqueous leaf extract of H. indicum can be explored at the ethnobotanical dose of 30 and 75 mg/kg b.wt on the management of some of the tissue-specific disarrays associated with diabetes.
Methods: Twenty-five rats were randomized into five groups. The study was carried out at the Animal Holding Unit, Biochemistry Department, University of Ilorin in 2013. Four groups were intraperitoneally administered singly with 150 mg/kg b.wt of alloxan to induce hyperglycemia. The normal control (NC) and hyperglycaemic control (HC) groups were administered 1 ml distilled water, while the reference group (HR) were administered 14.2 mg/kg b.wt of metformin and the test groups, H30 and H75 were administered 30 and 75 mg/kg b.wt, the extract respectively for fourteen days.
Results: The significantly increased (P<0.05) serum concentrations of tissue membrane bound enzymes (ALT, AST, ACP and ALP), direct and total bilirubin, urea and creatinine in HC indicating compromised tissue structures and functions in HC were attenuated. The significantly (P<0.05) reduced serum total protein, globulin and albumin in HC were significantly increased by both doses of the extract. The ameliorative role of the extract at the test doses was supported by the histological assessment of liver and kidney of the animals.
Conclusion: Aqueous leaf extract of H. indicum can be explored at the ethnobotanical dose of 30 and 75 mg/kg b.wt on the management of some of the tissue-specific disarrays associated with diabetes.
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