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Volume 12, Issue 3 (May-June 2018)
IJT 2018, 12(3): 9-13 |
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Bahtouee M, Fatemikia H, Movahed A, Esmaili A, Hassan Y, Zarei M et al . A Comparative Analysis of Saffron and Methylprednisolone on Bleomycin-Induced Pulmonary Fibrosis in Rats. IJT 2018; 12 (3) :9-13
URL: http://ijt.arakmu.ac.ir/article-1-674-en.html
URL: http://ijt.arakmu.ac.ir/article-1-674-en.html
Mehrzad Bahtouee1 
, Hossein Fatemikia2 , Ali Movahed3 , Abdolhamid Esmaili4 , Yaghoob Hassan5 , Mohammad Zarei1 , Ramin Seyedian * 6
, Hossein Fatemikia2 , Ali Movahed3 , Abdolhamid Esmaili4 , Yaghoob Hassan5 , Mohammad Zarei1 , Ramin Seyedian * 6
1- Department of Internal Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
2- Departments of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
3- PhD of Biochemistry, Persian Gulf Tropical Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.
4- Department of Pathology, Bushehr University of Medical Sciences, Bushehr, Iran.
5- BSc of Nutrition, Student Research Committee Center, Bushehr University of Medical Sciences, Bushehr, Iran.
6- Department of Pharmacology, Bushehr University of Medical Sciences, Bushehr, Iran. , raminseyedian@gmail.com
2- Departments of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
3- PhD of Biochemistry, Persian Gulf Tropical Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.
4- Department of Pathology, Bushehr University of Medical Sciences, Bushehr, Iran.
5- BSc of Nutrition, Student Research Committee Center, Bushehr University of Medical Sciences, Bushehr, Iran.
6- Department of Pharmacology, Bushehr University of Medical Sciences, Bushehr, Iran. , raminseyedian@gmail.com
Abstract: (3411 Views)
Background: The purpose of this study was to compare the effects of saffron and methylprednisolone on bleomycin-induced pulmonary fibrosis in rats.
Methods: This study was conducted in Bushehr, southern Iran in 2017.The animals were divided into four groups of five rats each. Three groups were injected with a single intratracheal dose of bleomycin (5 mg/kg). The fourth group was administered with normal saline at the same volume (200 µl). Saffron extract dissolved in water was given to one group (100 mg /body weight) orally while intraperitoneal injection of methylprednisolone (2.5 mg/kg) injected to another one for 16 days. The rats were sacrificed 28 days following surgery and their right and left lungs were removed and washed for measuring lung indices, myeloperoxidase activities and finally histopathological examination.
Results: Injection of bleomycin caused decrement of body weight aggravated by intraperitoneal methylprednisolone treatment. Lung indices were increased in the bleomycin-treated group compared with the control, while methylprednisolone, unlike saffron, had no preventive effects on it. Both saffron and methylprednisolone treatment prevented the increase in lung myeloperoxidase as a destructive enzyme. In addition, excessive collagen deposition and thickening of alveolar septa were significantly prevented with saffron treatment as compared to methylprednisolone injection following hematoxylin and eosin staining.
Conclusion: Saffron with established antioxidant properties could prevent some detrimental effects in bleomycin-induced pulmonary fibrosis even more than methylprednisolone injection known as a standard therapy in this murine model. More investigations must be carried out to examine the beneficial or harmful effects of this remedy.
Methods: This study was conducted in Bushehr, southern Iran in 2017.The animals were divided into four groups of five rats each. Three groups were injected with a single intratracheal dose of bleomycin (5 mg/kg). The fourth group was administered with normal saline at the same volume (200 µl). Saffron extract dissolved in water was given to one group (100 mg /body weight) orally while intraperitoneal injection of methylprednisolone (2.5 mg/kg) injected to another one for 16 days. The rats were sacrificed 28 days following surgery and their right and left lungs were removed and washed for measuring lung indices, myeloperoxidase activities and finally histopathological examination.
Results: Injection of bleomycin caused decrement of body weight aggravated by intraperitoneal methylprednisolone treatment. Lung indices were increased in the bleomycin-treated group compared with the control, while methylprednisolone, unlike saffron, had no preventive effects on it. Both saffron and methylprednisolone treatment prevented the increase in lung myeloperoxidase as a destructive enzyme. In addition, excessive collagen deposition and thickening of alveolar septa were significantly prevented with saffron treatment as compared to methylprednisolone injection following hematoxylin and eosin staining.
Conclusion: Saffron with established antioxidant properties could prevent some detrimental effects in bleomycin-induced pulmonary fibrosis even more than methylprednisolone injection known as a standard therapy in this murine model. More investigations must be carried out to examine the beneficial or harmful effects of this remedy.
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The Iranian Journal of Toxicology
Arak University of Medical Sciences in collaboration with the Iranian Society of Toxicology
Website: http://ijt.arakmu.ac.ir
Email: tox.journal@gmail.com
