Volume 15, Issue 1 (January 2021)
IJT 2021, 15(1): 1-8 |
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Emad Soleimani1 
, Hossein Sarmadian2 , Mohammad Arjomand Zadegan3 , Reza Ghasemikhah * 4, Hasan Taher Ahmadi5
, Hossein Sarmadian2 , Mohammad Arjomand Zadegan3 , Reza Ghasemikhah * 4, Hasan Taher Ahmadi5
1- Student Research Committee, Arak University of Medical Sciences, Arak, Iran.
2- Department of Infectious Disease, School of Medicine, Arak University of Medical Sciences. Arak, Iran.
3- Department of Microbiology, Infectious Diseases Research Center (IDRC), School of Medicine, Arak University of Medical Sciences, Arak, Iran.
4- Department of Pediatrics, School of Medicine, Arak University of Medical Sciences, Arak, Iran. , ghasemikhah@yahoo.com
5- Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
2- Department of Infectious Disease, School of Medicine, Arak University of Medical Sciences. Arak, Iran.
3- Department of Microbiology, Infectious Diseases Research Center (IDRC), School of Medicine, Arak University of Medical Sciences, Arak, Iran.
4- Department of Pediatrics, School of Medicine, Arak University of Medical Sciences, Arak, Iran. , ghasemikhah@yahoo.com
5- Department of Parasitology and Mycology, School of Medicine, Arak University of Medical Sciences, Arak, Iran.
Abstract: (2437 Views)
Background: Hydatidosis is a common disease of both humans and animals, resulting from infection with the larvae of Echinococcus granulosus tapeworm. The aim of this study was to investigate the antiparasitic (protoscolicidal) activities of three essential oils in vitro.
Methods: This study was designed to evaluate the biochemical composition and in vitro antiparasitic effects of Zataria multiflora, Origanum vulgare and Mentha pulegium essential oils. Gas chromatography was performed to identify the main components of the herbal oils. To determine the antiparasitic properties of the essential oils, live protoscoleces from hydatid cysts were exposed to three concentrations of the herbal oils and were incubated at 37°C for 5, 10, 15, 20 or 25 minutes.
Results: The biochemical analysis of these oils indicated that carvacrol and thymol were the major compounds of the Zataria oil. Further, carvacrol and thymol in Origanum essential oil and pulegone and piperitone in Mentha oil were the major compounds. The quickest and slowest antiparasitic effect was achieved from Zataria and Origanum (10%) or from Zataria (0.6%), respectively. The statistical analysis showed a significant difference between the mortality rate of protoscoleces exposed to 0.6% and 1% concentrations, respectively, of Zataria and Origanum at the predetermined exposure times (P<0.05). The three concentrations of Mentha had the same significant statistical differences (P<0.05).
Conclusion: Essential oils, Zataria multiflora, Origanum vulgare and Mentha pulegium had significant protoscolicidal activities that were dependent on the concentration of the oils and the exposure times.
Methods: This study was designed to evaluate the biochemical composition and in vitro antiparasitic effects of Zataria multiflora, Origanum vulgare and Mentha pulegium essential oils. Gas chromatography was performed to identify the main components of the herbal oils. To determine the antiparasitic properties of the essential oils, live protoscoleces from hydatid cysts were exposed to three concentrations of the herbal oils and were incubated at 37°C for 5, 10, 15, 20 or 25 minutes.
Results: The biochemical analysis of these oils indicated that carvacrol and thymol were the major compounds of the Zataria oil. Further, carvacrol and thymol in Origanum essential oil and pulegone and piperitone in Mentha oil were the major compounds. The quickest and slowest antiparasitic effect was achieved from Zataria and Origanum (10%) or from Zataria (0.6%), respectively. The statistical analysis showed a significant difference between the mortality rate of protoscoleces exposed to 0.6% and 1% concentrations, respectively, of Zataria and Origanum at the predetermined exposure times (P<0.05). The three concentrations of Mentha had the same significant statistical differences (P<0.05).
Conclusion: Essential oils, Zataria multiflora, Origanum vulgare and Mentha pulegium had significant protoscolicidal activities that were dependent on the concentration of the oils and the exposure times.
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