TY - JOUR JF - IJT JO - IJT VL - 10 IS - 1 PY - 2016 Y1 - 2016/1/01 TI - Effects of Short-Term Exposure to Sublethal Concentrations of Silver Nanoparticles on Histopathology and Electron Microscope Ultrastructure of Zebrafish (Danio Rerio) Gills TT - Effects of Short-Term Exposure to Sublethal Concentrations of Silver Nanoparticles on Histopathology and Electron Microscope Ultrastructure of Zebrafish (Danio Rerio) Gills N2 - Background: The increasing use of nanomaterials and nanoproducts has increased the possibility of contamination of the environment, which may have adverse effects on different organisms. The aim of this study was to evaluate the effects of silver nanoparticles on histopathology and gill ultrastructure of zebrafish (Danio rerio) under laboratory conditions. Methods: Zebrafish were exposed to four concentrations of silver nanoparticles (0.0015, 0.00375, 0.0075, and 0.015 mg/l) for a period of 4 days. Gill ultrastructure and histopathological changes were studied using scanning electron microscope and haematoxylin - eosin staining. Results: Exposure to silver nanoparticles significantly (P < 0.001) increased the diameter of gill filaments and secondary lamellae, while silver nanoparticles significantly reduced the length of the secondary gills in zebrafish. Moreover, other changes such as vacuolization, dilated and clubbed tips, hyperplasia, edema, fusion, swelling of mucocytes, hypertrophy, and necrosis were observed. The effects of silver nanoparticles in zebrafish gills were dose dependent. Conclusion: Based on the adverse effects of AgNPs on zebrafish gills, silver nanoparticle solutions can be hazardous pollutants for the environment. SP - 15 EP - 20 AU - Mansouri, Borhan AU - Johari, Seyed Ali AD - PhD Student of Ecotoxicology, Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran. KW - Gills KW - Metal Nanoparticles KW - Silver KW - Water Pollution KW - Zebrafish. UR - http://ijt.arakmu.ac.ir/article-1-409-en.html DO - 10.32598/IJT.10.1.60.4 ER -