Iranian Journal of Toxicology
مجله سم شناسی و مسمومیتهای ایران
IJT
Medical Sciences
http://ijt.arakmu.ac.ir
1
admin
2008-2967
2251-9459
8
10.61186/ijt
14
8888
13
en
jalali
1401
2
1
gregorian
2022
5
1
16
2
online
1
fulltext
en
Protective Effect of Trehalose Against H2O2-induced Cytotoxicity and Oxidative Stress in PC-12 Cell Line and the Role of Heat Shock Protein-27
عمومى
General
پژوهشي
Research
<strong>Background: </strong>Oxidative stress has been shown to be an important factor, which plays a significant role in the pathogenesis of neurodegenerative disorders. Heat Shock Protein-27 (HSP-27) has been implicated in antioxidant responses against oxidative stress. Trehalose is a natural disaccharide widely used in a variety of food products with demonstrated protective effects against several neurodegenerative diseases. This study investigated the effects of trehalose on antioxidant responses, and the gene expressions for HSP-27 and caspase-3 against hydrogen peroxide (H2O2) induced oxidative injury in PC-12 cell line. <br>
<strong>Methods: </strong>The PC-12 cells were treated with various concentrations of H2O2 and trehalose for 24hr. The cell viability was assessed, using MTT and Lactate Dehydrogenase (LDH) release assays. Moreover, the activity of Catalase (CAT) and Glutathione Peroxidase (GPx) enzymes, and the Malondialdehyde (MDA) levels were determined. In addition, the levels of HSP-27 and caspase-3 gene expressions were measured. <br>
<strong>Results:</strong> The results indicated that the pretreatment with trehalose increased cell survival against the H2O2-induced oxidative injury. Furthermore, trehalose elevated the CAT and GPx activities and reduced MDA levels compared to that of control group (P˂0.05). Moreover, trehalose upregulated the HSP-27 gene expression, while reducing the expression of caspase-3 gene compared to that of the untreated cells (P˂0.05). All of these biochemical changes were found to be dose-dependent for trehalose. <br>
<strong>Conclusion: </strong>Based on the study findings, trehalose had the capacity to attenuate the oxidative stress and cell injury. Therefore, trehalose may be suggested as a therapeutic agent to treat neurodegenerative disorders caused by oxidative stress damages.<br>
Antioxidant, Cell viability, HSP27, Oxidative injury, Trehalose
145
152
http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-905-1&slc_lang=en&sid=1
Akram
Norouzi
a.norouzi6886@yahoo.com
100319475328460012985
100319475328460012985
No
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Nasrin
Ziamajidi
n.ziamajidi@umsha.ac.ir
100319475328460012986
100319475328460012986
No
Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Asieh
Sadeghi
a.sadeghi@kmu.ac.ir
100319475328460012987
100319475328460012987
No
Department of Clinical Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.
Mahdieh
Nazari-Robati
mnazari@kmu.ac.ir
100319475328460012988
100319475328460012988
Yes
Department of Clinical Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran.