en The Cytotoxic and Synergistic Effects of Flavonoid Derivatives on Doxorubicin Cytotoxicity in Hela, MDA-MB-231, and HT-29 Cancer Cells عمومى General پژوهشي Research <b>Background: </b>Flavonoids have a variety of biological activities, such as anti-allergic, anti-inflammatory, anti-oxidative, free radical scavenging, and anti-mutagenic. <br><b>Methods: </b>The cytotoxic effects of three synthesized flavonoid derivatives (K3, K4 and K5) were evaluated against Hela , MDA-MB -231 and HT-29 cancer cells using MTT assay. <br><b>Results: </b>The results showed that these flavonoids were not cytotoxic at any tested concentrations (0.1, 0.05, 0.01, and 0.001 mM). To evaluate the possible synergistic effect of synthetic flavonoids with chemotherapeutic agent, the compound (K4) was examined against Hela and MDA-MB-231 cells in combination with different concentrations of doxorubicin (0.01 and 0.001mM). This combination treatment significantly increased the cytotoxicity of doxorubicin (P < 0.05). <br><b>Conclusion: </b>Synthesized flavonoids could be used either in combination therapy with other chemotherapeutic agents or used as antioxidants in food supplements. Hela, HT-29, MDA-MB-231, MTT Assay, Synergism, Synthetic Flavonoid. Hela, HT-29, MDA-MB-231, MTT Assay, Synergism, Synthetic Flavonoid. 558 564 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-2-81&slc_lang=en&sid=1 Hojjat Sadeghi-Aliabadi حجت صادقی علی آبادی sadeghi@pharm.mui.ac.ir 10031947532846002978 10031947532846002978 Yes School of Pharmacy and Pharmaceutical Sciences, Isfahan, Iran. School of Pharmacy and Pharmaceutical Sciences, Isfahan, Iran. Hossein Mosavi حسین موسوی 10031947532846002979 10031947532846002979 No Isfahan University of Medical Sciences, Isfahan, Iran. Isfahan University of Medical Sciences, Isfahan, Iran. Mina Mirian مینا میریان 10031947532846002980 10031947532846002980 No Isfahan University of Medical Sciences, Isfahan, Iran. Isfahan University of Medical Sciences, Isfahan, Iran. Saeed Kakhki سعید کاخکی 10031947532846002981 10031947532846002981 No Shahid Beheshti University of Medical Sciences, Tehran, Iran. Shahid Beheshti University of Medical Sciences, Tehran, Iran. Afshin Zarghi افشین زرقی 10031947532846002982 10031947532846002982 No Shahid Beheshti University of Medical Sciences, Tehran, Iran. Shahid Beheshti University of Medical Sciences, Tehran, Iran.