en عمومى General پژوهشي Research <div style="text-align: justify;"><span style="font-size:12px;"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-GB">Background: </span></b><i><span lang="EN-GB">Acacia aroma </span></i><span lang="EN-GB">(<i>A. aroma</i>), or Tusca, is a South American plant</span> frequently used in medicine<span lang="EN-GB">.</span> We recently reported the antiulcer effect of two Tusca leaf extracts in rats<span lang="EN-GB">: a 5% infusion and a 10% hydroalcoholic. This study aimed to investigate the <i>in vitro</i> cytotoxicity and the <i>in vivo</i> oral subchronic toxicity of these two extracts in rats. </span></span></span><br> <span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-GB">Methods: </span></b><span lang="EN-GB">C</span><span lang="EN-GB">ytotoxicity was evaluated through cell viability assays using the 50% lethal concentration (LC₅₀) on lung cells, which was calculated for each extract. Oral subchronic toxicity was studied in Wistar rats, and the clinical parameters were determined during a 90-day treatment period. Following the experimental period, biochemical parameters were analyzed, and histopathological analyses of the stomach, liver, and kidneys were performed.</span></span></span><br> <span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-GB">Results: </span></b><span lang="EN-GB">The <i>in vitro</i> cytotoxicity was low in both extracts (LC₅₀=966.78&plusmn;48.34 &micro;g/ml for the 5% infusion and 562.28&plusmn;28.11 &micro;g/ml for the 10% hydroalcoholic extract). The <i>in vivo</i> clinical, behavioral, and biochemical parameters, body weight, and food intake had no significant differences (P&ge;0.05) compared to the control group. There were also no histopathological alterations in the analyzed organs.</span><span style="font-size:8.0pt"></span></span></span><br> <span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-GB"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b>The present study contributes to expanding the knowledge on the safety of the two extracts derived from <i><span lang="EN-GB">A. aroma</span></i><i> </i>(5% infusion and 10% <span lang="EN-GB">hydroalcoholic</span>). Therefore, the extracts can possibly be used in the treatment or prevention of gastric diseases in future studies.</span></span></span></div> Acacia aroma, Cytotoxic activity, Preclinical study, Subchronic toxicity, Tusca leaf extracts 71 77 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1182-1&slc_lang=en&sid=1 Félix F. Taboada felix.taboada@fbqf.unt.edu.ar 100319475328460016596 100319475328460016596 No Institute of Biology, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán. Superior Institute of Biological Research, National Council of Scientific and Technical Research, Chacabuco 461, San Miguel de Tucumán, Argentina Natalia C. Habib natalia.habib@fbqf.unt.edu.ar 100319475328460016597 100319475328460016597 No Institute of Biology, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán. Superior Institute of Biological Research, National Council of Scientific and Technical Research, Chacabuco 461, San Miguel de Tucumán, Argentina Susana B. Genta susana.genta@fbqf.unt.edu.ar 100319475328460016598 100319475328460016598 Yes Institute of Biology, Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán. Superior Institute of Biological Research, National Council of Scientific and Technical Research, Chacabuco 461, San Miguel de Tucumán, Argentina