en عمومى General پژوهشي Research <span style="font-size:12px;"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-GB">Background: </span></b><span lang="EN-GB">Cancer occurs in 83% of liver diseases. Other risk factors for liver cancer include viral hepatitis, alcohol consumption, industrial chemicals, and a number of toxins. Another major disease that occurs following liver damage is hepatic encephalopathy. This condition arises primarily due to increased blood ammonia levels. Carvacrol, with antioxidant properties, reduces oxidative stress on the liver. The aim of this study was to investigate the effect of carvacrol on the improvement of hepatic encephalopathy in rats.</span></span></span><br> <span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-GB">Methods: </span></b><span lang="EN-GB">In this experimental study, 60 male Wistar rats were randomly divided into six groups of 10 each. Liver damage and induction of oxidative stress were caused in the rats by administering thioacetamide (100 mg/kg/day) intraperitoneally for three consecutive days. Carvacrol was administered by gavage at 25, 50, or 100 mg/kg/day after thioacetamide treatment. We investigated the biomarkers of liver damage in the blood, such as alanine transaminase, lactate dehydrogenase, total protein, and bilirubin. We also assessed the effect of oxidative stress, as the key inducer of hepatic encephalopathy, on the liver by measuring the lipid peroxidation, antioxidants, reactive oxygen species, glutathione reserves, and ammonium levels in the serum and brain.</span></span></span><br> <span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-GB">Results: </span></b><span lang="EN-GB">Thioacetamide significantly increased the biochemical markers in the rat sera, reflecting ammonium release and the development of oxidative stress (P<0.05). Conversely, the various doses of carvacrol significantly reduced the levels of biomarkers that are indicative of liver damage (P<0.05).</span></span></span><br> <span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span lang="EN-GB"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b><span lang="EN-GB">The study findings provided experimental evidence in favor of the therapeutic effects of carvacrol and against liver injury induced by thioacetamide, leading to encephalopathy.</span></span></span></span><span style="font-size:12pt"><span style="line-height:normal"><span new="" roman="" style="font-family:" times=""><b><span lang="AR-SA" dir="RTL" style="font-size:8.0pt"></span></b></span></span></span> Ammonium, Carvacrol, Hepatic encephalopathy, Oxidative stress, Thioacetamide liver damage 144 151 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1209-1&slc_lang=en&sid=1 Anahita Esmaeili Anahitaa.esmaeilii@gmail.gmail.com 100319475328460017881 100319475328460017881 No Pharmacy Student, Student Research Committee, Lorestan University of Medical Sciences. Khoram-Abad, Iran. Peyman Astaraki Peymanastaraki@yahoo.com 100319475328460017882 100319475328460017882 No Department of Internal Medicine, School of Medicine, Shahid Rahimi Hospital, Lorestan University of Medical Sciences, Khoram-Abad, Iran. Mohammad Jamalian 100319475328460017883 100319475328460017883 No Department of Forensic Medicine, School of Medicine, Valiasr Hospital, Arak University of Medical Sciences, Arak, Iran. Hamidreza Mohammadi hamidrezamohammadi65@yahoo.com 100319475328460017884 100319475328460017884 Yes Department of Toxicology, Faculty of Pharmacy, Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khoram-Abad, Iran. Naser Reza Ziyaeenia 100319475328460017885 100319475328460017885 No Department of Health Education, School of Medicine, Lorestan University of Medical Sciences, Khoram-Abad, Iran