en عمومى General پژوهشي Research <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Background:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Colon cancer is one of the most prevalent malignancies in both men and women. This study investigated the anticancer efficacy of a new synthetic form of quinolone nanoparticles (QNPs) against colon cancer cells. As an experimental model, we investigated methods to prevent the development of colon cancer in adult male albino rats induced by 1,2-dimethylhydrazine </span></span></span><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">(DMH).</span></span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Methods:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Forty adult male rats (weighing 150-200 g) were randomly assigned to four groups of ten as follows: </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Group 1:</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Normal rats considered the reference group. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Group 2:</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Normal rats treated intraperitoneally with 100 &mu;g/kg/day of quinolone. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Group 3: </span></span></span></i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Rats with induced colon cancer by DMH. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Group 4:</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> Rats with colon cancer, as the experimental model, and administered QNPs at 100 &mu;g/kg/day for 6 weeks.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> The results showed that QNPs significantly improved the treatment of induced colon cancer in rats. This finding was supported by significant increases in CD4, colon SOD, and GPx activity, as well as in GSH and CAT levels. Further discoveries included a significant decline in the serum values of CEA, CA19-9, AFP, TNF-&alpha;, IL1&beta;, ALT, AST, urea, creatinine, cholesterol, triglycerides, colon DNA damage, MDA, and NO. The histopathological results demonstrated the therapeutic potential of QNPs, which were successful in halting the development of colon cancer in an experimental animal model. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-justify:inter-ideograph"><span style="line-height:115%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Conclusions:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""> The findings of the current study demonstrated that QNPs were able to prevent colon cancer in rats by enhancing their immune system, lowering inflammatory markers, and improving damaged oxidative stress tolerance.</span></span></span></span></span></span></span> Anticancer, Colon cancer, Dimethylhydrazine (DMH), Nanoparticles, Quinolone 166 181 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1122-4&slc_lang=en&sid=1 Asmaa Mohammed Attya asmaaattya@gmail.com 100319475328460016705 100319475328460016705 No Zoology Dept, Faculty of Science, Al-Azhar University, Assuit, Egypt Mahmoud Ashry mahmoud_ashry20@yahoo.com 100319475328460016706 100319475328460016706 Yes Zoology Dept, Faculty of Science, Al-Azhar University, Assuit, Egypt Abdelbaset M.A. Abdelreheem basetabdelreheem@yahoo.com 100319475328460016707 100319475328460016707 No Zoology Dept, Faculty of Science, Al-Azhar University, Assuit, Egypt Doaa Galal El-Sahra : basetabdelreheem@yahoo.com 100319475328460016708 100319475328460016708 No Modern University for Technology and Information, Cairo, Egypt. Mohammed A. Abdel-Aal drdoaagalal@yahoo.com 100319475328460016709 100319475328460016709 No Dept. of Pharmaceutical Chemistry, Faculty of Pharmacy, Al-Azhar University, Assuit, Egypt.