en تخصصي Special پژوهشي Research <span style="font-size:12px;"><span style="font-family:Tahoma;"><b><span style="color:#2d73b4">Background: </span></b>This study aimed to investigate the effects of betaine on acrylamide (ACR)-induced damage in the liver and kidneys of rats.<br> <b><span style="color:#2d73b4">Methods: </span></b>A total of 28 male rats were divided into four groups. The first group served as the control. The second group received ACR (50 mg/kg body weight; i.p.) during the experiment (11 days). Group 3 received betaine (2% in diet) and ACR. Group 4 rats received betaine (2% in diet).<br> <b><span style="color:#2d73b4">Results: </span></b>Plasma alanine aminotransferase (ALT), aspartate aminotransferase, urea, creatinine, and bilirubin values increased significantly in Group 2 compared to the control group. Betaine treatment in Group 3 significantly decreased ALT values relative to Group 2. Hepatic glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH), as well as renal GPx, SOD, fluorescence recovery after photobleaching (FRAP), and GSH values, showed a significant decline in Group 2 than in the controls. The hepatic and renal GPx, SOD, CAT, FRAP, and GSH levels in Group 3 notably increased to levels comparable to those of the control group. Additionally, a significant ascending trend in tissue protein carbonyl and malondialdehyde levels was observed in Group 2. In contrast, betaine treatment in the third group decreased these indices to levels comparable to those of the controls. Moreover, ACR-induced histopathological features, including a significant increase in hepatic hyperemia and necrosis, renal necrosis, and cell swelling, were attenuated by betaine treatment in Group 3.<br> <b><span style="color:#2d73b4"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b>These findings indicate that betaine can mitigate ACR-induced hepatotoxicity and nephrotoxicity, which may be related to betaine&#39;s antioxidant and methyl-donor potential.</span></span><br> <br> &nbsp; Acrylamide (ACR), Antioxidant, Betaine, Kidney, Liver 248 258 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-359-3&slc_lang=en&sid=1 Javad Fasahi javadfasahi71@gmail.com 100319475328460018783 100319475328460018783 No Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Hasan Baghshani baghishani@um.ac.ir 100319475328460018784 100319475328460018784 Yes Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Zahra Moosavi zmoosavi@um.ac.ir 100319475328460018785 100319475328460018785 No Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.