en عمومى General پژوهشي Research <span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="line-height:200%"><b><span style="line-height:200%">Background:</span></b><span style="line-height:200%"> Formalin, as formaldehyde dissolved in water, is a carcinogen that causes oxidant and antioxidant imbalance. The metabolic products of formaldehyde, such as formic acid, stimulate oxidative stress, leading to kidney damage. <i>Bidens pilosa</i> L. (<i>B. pilosa</i> L.) leaves contain flavonoids with antioxidant and anti-inflammatory activities. The present study aimed to determine the nephroprotective effect of <i>B. pilosa</i> L. leaf extract on the histological structure and weight of the kidney organs of white rats (<i>Rattus norvegicus</i>) induced by formalin.</span></span><br> <span style="line-height:200%"><b><span style="line-height:200%">Methods:</span></b><span style="line-height:200%"> Rats were grouped into five treatment groups, including normal control, negative control, and three dose groups (25 mg/kg BW, 50 mg/kg BW, and 100 mg/kg BW). Rats were induced with formalin 0.2 ml/kg BW for 7 days orally, followed by dose treatment for 7 days. Kidney histology and weight were examined on day 14. Antioxidant and anti-inflammatory activities were predicted by molecular docking.</span></span><br> <span style="line-height:200%"><b><span style="line-height:200%">Results:</span></b><span style="line-height:200%"> The kidney weight in the treatment was not significantly different, but histologically, the <i>B. pilosa</i> L. leaf extract could significantly reduce necrosis cells at a dose of 100 mg/kg to approach the score of the normal group. Secondary metabolites, such as isochlorogenic acid and luteolin, had antioxidant and anti-inflammatory properties, which contribute to therapy.</span></span><br> <span style="line-height:200%"><b><span style="line-height:200%">Conclusion:</span></b><span style="line-height:200%"> <i>B. pilosa</i> L. leaf extract can potentially protect nephrons with antioxidant and anti-inflammatory activities.</span></span></span></span> Bidens pilosa L., Formaldehyde, Formalin, Kidney, Rat 70 75 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1335-1&slc_lang=en&sid=1 Muhyiddin Fathan Azzami masfathazz@student.uns.ac.id 100319475328460017439 100319475328460017439 No Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Surakarta, Indonesia Okid Parama Astirin parama_astirin@staff.uns.ac.id 100319475328460017440 0009-0002-8327-4711 Yes Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Surakarta, Indonesia Shanti Listyawati shantilistyawati@staff.uns.ac.id 100319475328460017441 100319475328460017441 No Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Surakarta, Indonesia Widya Mega Rahmawati widyamegarahmawati@student.uns.ac.id 100319475328460017442 100319475328460017442 No Department of Biology, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Surakarta, Indonesia