en تخصصي Special پژوهشي Research <span style="font-size:12px;"><span style="font-family:Tahoma;"><span style="line-height:103%"><b><span style="line-height:103%">Background<span style="color:#2c73b4">: </span></span></b><span style="line-height:103%">Avocado peels (Persea americana Mill.) contain polyphenolic compounds that can be used as alternative medicinal plants, such as anti-inflammatory, antibacterial, and antifungal agents. This research aims to determine the acute toxicity of ethanol extract from avocado peels on ddY strain mice.</span></span><br> <span style="line-height:103%"><b><span style="line-height:103%">Methods: </span></b><span style="line-height:103%">This experimental research using Acute Toxic Class Method (OECD 423) was conducted on 15 female mice ddY strain that were divided into five groups with one control group (CG) and four treatment groups. The four treatment groups administrated by ethanolic extract of avocado peel at Group 1 (G1) with 300 mg/kgBW single dose, Group 2 (G2) with 300 mg/kgBW repeated dose, Group 3 (G3) with 2,000 mg/kgBW single dose, and Group 4 (G4) with 2,000 mg/kgBW repeated dose were observed for 14 days.<br> <b>Results:<span style="color:#2c73b4"> </span></b>The results showed that avocado peels did not cause any toxic symptoms, including mortality (0%). No behavioral abnormalities or macroscopic changes in organ color, size, and relative organ weight were observed. Statistical analysis revealed no significant differences in the relative liver and kidney weight between the control and treatment groups (P>0.05). Although a reduction in body weight was noted, it was not statistically significant (P>0.05)</span></span><br> <span style="line-height:103%"><b><span style="line-height:103%">Conclusion: </span></b><span style="line-height:103%">All doses of the ethanolic extract of avocado peels fall into the practically non-toxic category (category 5) based on Globally Harmonised System (GHS).</span></span></span></span> Acute toxicity test, Avocado peel, Behavioral changes, Body weight, Macroscopic changes, Mortality 98 106 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1347-1&slc_lang=en&sid=1 Welly Ratwita wellyratwita@gmail.com, welly.ratwita@lecture.unjani.ac.id 100319475328460017777 000900090761405 No Pharmacology, Department of Pharmacology, School of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia Afni Mayzida afniyuafi@gmail.com 100319475328460017778 0009000146324110 No Pharmacology, Department of Pharmacology, School of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia Aninditha Dewi anindithalaras90@gmail.com 100319475328460017779 0009000412559909 Yes Pharmacology, Department of Pharmacology, School of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia Nafisa Kaffah nafisasilmik@gmail.com 100319475328460017780 0009000966331527 No Pharmacology, Department of Pharmacology, School of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia Nurfadhiya Maharani previmhr@gmail.com 100319475328460017781 100319475328460017781 No Pharmacology, Department of Pharmacology, School of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia Fransiska Pontjosudargo ambarukmifransiska@gmail.com, fransiska.ambarukmi@lecture.unjnai.ac.id 100319475328460017782 100319475328460017782 No Anatomy, Department of Anatomy, School of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia Evy Shavilla evy.shavilla@gmail.com 100319475328460017783 100319475328460017783 No Ear, Nose, and Throat Specialist, Department of Ear, Nose, and Throat, School of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia Aprilia Sweetasari 100319475328460017784 100319475328460017784 No Neurologist, Department of Neurologi, School of Medicine, Jenderal Achmad Yani University, Cimahi, Indonesia