en عمومى General پژوهشي Research <p style="text-align:justify"><span style="font-size:12px;"><span style="font-family:Tahoma;"><b><span style="color:#2d73b4">Background: </span></b><i>Mucuna pruriens</i> and<i> Justicia carnea</i> are plants with known therapeutic properties used in traditional medicine to treat a range of ailments. The present study aimed to examine the effects of <i>Mucuna pruriens</i>, <i>Justicia carnea</i>, and their combined ethanol leaf extracts on the biochemical indices of anemic rats induced by phenylhydrazine.<br> <b><span style="color:#2d73b4">Methods: </span></b>Thirty male albino rats were divided into six groups of five rats each. Anemia was induced using 80 mg/kg phenylhydrazine intraperitoneally, except in the normal control. Group 1 served as normal control, Group 2 as anemic control, and Group 3 received Astyfer (1.5 mg/kg). Groups 4-6 were treated with 200 mg/kg of <i>Mucuna pruriens</i>, <i>Justicia</i> <i>carnea</i>, and their combination. Treatments were given orally for three weeks before blood collection for biochemical analysis.<br> <b><span style="color:#2d73b4">Results: </span></b>Liver function test revealed a significant increase in Alkaline phosphatase level of groups 3 and 6 (p<0.05). Kidney function test showed a significant increase in potassium level in group 3 and urea level in groups 2 and 6 (p<0.05), while creatinine level significantly decreased in groups 3, 5, and 6 (p<0.05). Lipid profile results showed a significant decrease in total cholesterol level of groups 2, 3, and 6 (p<0.05). Antioxidant assay showed no significant differences (p>0.05), except for a significant decrease (p<0.05) in catalase level in group 5.<br> <b><span style="color:#2d73b4"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b>Findings from this study indicated that <i>Mucuna pruriens</i>, <i>Justicia carnea,</i> and their combined extract have a potent protective effect against complications arising from anemia, as the extracts restored most of the altered biochemical markers towards normal.<br> &nbsp;</span></span></p> Anemia, Biochemical, Justicia carnea, Mucuna pruriens, Phenylhydrazine 167 172 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-989-1&slc_lang=en&sid=1 Chikezie Kenneth Ogbu chikezie_ken@hotmail.com 100319475328460018863 0009-0000-9087-3718 No Department of Applied Biochemistry, Enugu State University of Science and Technology, Agbani, Nigeria. Onuabuchi Nnenna Ani nnenna.ani@esut.edu.ng 100319475328460018864 100319475328460018864 Yes Department of Applied Biochemistry, Enugu State University of Science and Technology, Agbani, Nigeria. Cosmas Ezekaibeya Achikanu cosmas.achikanu@esut.edu.ng 100319475328460018865 100319475328460018865 No Department of Applied Biochemistry, Enugu State University of Science and Technology, Agbani, Nigeria. Innocent Izuchukwu Ujah innocent.ujah@esut.edu.ng 100319475328460018866 100319475328460018866 No Department of Applied Biochemistry, Enugu State University of Science and Technology, Agbani, Nigeria. Ebere Immaculata Akpata ebere.akpata@esut.edu.ng 100319475328460018867 100319475328460018867 No Department of Applied Biochemistry, Enugu State University of Science and Technology, Agbani, Nigeria.