en عمومى General پژوهشي Research <p><span style="font-size:12px;"><span style="font-family:Tahoma;"><b><span style="color:#2d73b4">Background: </span></b>Polyphenolic compounds in buckwheat (<i>Fagopyrum esculentum</i>) are known for their antioxidant and detoxifying properties. Among them, rutin is a major flavonoid with potential biological activity. The present study focused on the comparative analysis of polyphenolic content in roasted and green buckwheat and explored rutin&rsquo;s interaction with sirtuin (SIRT), a key protein involved in cellular stress responses and longevity.<br> <b><span style="color:#2d73b4">Methods: </span></b>High-performance liquid chromatography (HPLC) was employed to quantify polyphenolic compounds in roasted and green buckwheat samples. In silico molecular docking was performed using CB-Dock2 to assess the binding affinity of rutin with the active site of SIRT.<br> <b><span style="color:#2d73b4">Results: </span></b>The HPLC results demonstrated that green buckwheat contained significantly higher levels of rutin compared to roasted buckwheat (1.09 mg/g vs. 0.5 mg/g). Molecular docking revealed a strong binding affinity between rutin and SIRT, supporting its potential role in modulating sirtuin-related protective pathways involved in detoxification.<br> <b><span style="color:#2d73b4"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b>Green buckwheat is a richer source of rutin, which may contribute to its enhanced detoxification potential. The strong in silico interaction between rutin and SIRT2&nbsp; suggests that rutin could serve as a natural modulator of cellular defense mechanisms, particularly those regulated by sirtuins.</span></span><br> &nbsp;</p> Detoxification, Fagopyrum, Molecular Docking Simulation, Polyphenols, Sirtuins Liver 207 212 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1410-1&slc_lang=en&sid=1 Nargiza Hamzaeva hamzagiza6@gmail.com 100319475328460018805 100319475328460018805 Yes Tashkent Institute of Chemical Technology, Department of Biotechnology, Tashkent, Uzbekistan. Uchkun Ishimov uchkunishimoc@gmail.com 100319475328460018806 0009-0001-9021-6782 No Institute of Bioorganic Chemistry of the Academy of Sciences of the Republic of Uzbekistan. Anvar Normatov anvarnormatov76@gmail.com 100319475328460018807 100319475328460018807 No Tashkent Institute of Chemical Technology, Department of Biotechnology, Tashkent, Uzbekistan.