en عمومى General پژوهشي Research <span style="font-size:12px;"><span style="font-family:Tahoma;"><b><span style="color:#2d73b4">Background: </span></b>Medicinal plants, including <i>Spilanthes filicaulis</i>, have been cornerstone treatments in the traditional primary healthcare system, mainly in tropical African countries. This versatile plant is extensively used to treat and manage various health conditions. In this regard, the present study aimed to amplify scientific evidence about the safety level of <i>Ethylacetate Subfraction</i> of <i>Spilanthes filicaulis</i> (ESSF).<br> <b><span style="color:#2d73b4">Methods: </span></b>A Toxicological study was conducted on 72 male Rattus norvegicus, divided into six groups (n=12) with weight ranges of 140-156 g. Group 1 served as the control group, receiving 10 ml/kg body weight distilled water daily, while Groups 2-6 received graded doses ranging from 62.50 to 750 mg/kg body weight of the extracts, respectively, once daily for 28 days. On days 14 and 28 after the treatment, rats underwent fasting for 12 h overnight and euthanized.&nbsp; Lipid profile and cellular enzymes tests (alanine aminotransferase [ALT], aspartate aminotransferase [AST], Gamma-Glutamyltransferase [GGT], Lactate Dehydrogenase [LDH], and Acetylcholine esterase [AchE]) were evaluated on prepared tissues (serum, brain, liver, and kidney). Histoarchitecture of vital organs was also done.<br> <b><span style="color:#2d73b4">Results: </span></b>The ESSF at most of the doses investigated on the rats did not significantly alter serum concentrations of lipid on days 14 and 28, compared to the control group (p>0.05). Additionally, the ESSF of all doses did not significantly alter the activities of ALT, AST, GGT, LDH, and AChE in most of the studied tissues on days 14 and 28, compared to the control group (p>0.05).<br> <b><span style="color:#2d73b4"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b>No abnormalities were found in the organs of treated animals during the histological analysis. Consequently, <i>R. norvegicus</i> treated with ESSF did not experience harmful effects.</span></span><br> <br> <div></div> Cellular enzymes, Lipid profile, Rattus norvegicus, Serum, Tissues 87 95 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1417-1&slc_lang=en&sid=1 Bankole Emmanuel Ofeniforo ofeniforo.bankole@bouesti.edu.ng 100319475328460018970 100319475328460018970 Yes Biochemistry Unit, Department of Chemical Sciences, Bamidele Olumilua, University of Education, Science and Technology, Ikere, Nigeria. Olalekan Bukunmi Ogunro olalekanbukunmi@gmail.com 100319475328460018971 100319475328460018971 No Biochemistry Unit, Department of Biological Sciences, KolaDaisi University, Ibadan, Nigeria. Johnson O. Oladele oladelejohn2007@gmail.com 100319475328460018972 100319475328460018972 No Royal Scientific Research Institute, Osun State, Nigeria. Samuel Eleojo Agada Samuelagada@tamu.edu 100319475328460018973 0009-0001-8730-6859 No Microbiome and Molecular Host-Pathogen Interactions Unit, Department of Poultry Science, Texas A&M University, Texas, USA. Oluwaseun Dorcas Ayo-Dada ayo-dada.oluwaseun@bouesti.edu.ng 100319475328460018974 100319475328460018974 No Public Health Unit, Department of Health Sciences, Bamidele Olumilua University of Education, Science and Technology, Ikere, Nigeria. Olukemi Omolade Ogunyebi ogunyebi.olukemi@bouesti.edu.ng 100319475328460018975 0009-0008-6149-6288 No Biochemistry Unit, Department of Chemical Sciences, Bamidele Olumilua University of Education, Science and Technology, Ikere, Nigeria Morenike Grace Ajayi ajayi.morenike@bouesti.edu.ng 100319475328460018976 0009-0008-0159-5735 No Chemistry (Analytical Chemistry) Unit, Department of Chemical Sciences, Bamidele Olumilua University of Education, Science and Technology, Ikere, Nigeria.