en عمومى General پژوهشي Research <span style="font-size:12px;"><span style="font-family:Tahoma;"><b><span style="color:#2d73b4">Background: </span></b>To investigate the effect of Ashwagandha (ASH) <i>Withania somnifera</i> (WS) in preventing the damage caused by Fluoxetine (FLX) on testis.<br> <b><span style="color:#2d73b4">Methods: </span></b>Adult thirty-two male Wistar rats were randomly divided into four groups (eight rats in each): a control group, an Ashwagandha-treated group (200 mg orally) according to Bhargavan et al (2015), a FLX -treated group (10 mg/kg orally), and a combined FLX and ASH-treated group. Hormonal profiles (testosterone, FSH, and LH) and sperm parameters (count, motility, abnormality, and semen fructose levels) were evaluated. Testicular tissues were analyzed for biochemical markers, including glutathione peroxidase (GSH), superoxide dismutase (SOD), glutathione S-transferase (GST), malondialdehyde (MDA), and nitric oxide (NO), as well as for histopathological changes.<br> <b><span style="color:#2d73b4">Results: </span></b>Biochemical parameters revealed tissue oxidative stress, in FLX group which was evidenced as increase in MDA, NO level and reduction in GSH, GST, SOD activities in testes which were reduced in co-treatment group. in addition, spermatogenesis was inhibited as indicated by the decrease (testosterone, LH and FSH), (sperm count, motility). Moreover, ASH treatment leads to an increase in testosterone (TS), LH, FSH levels, sperm count and motility.<br> <b><span style="color:#2d73b4"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b>These observations suggest that the antioxidant properties of Ashwagandha may have played a role in ameliorating the testicular toxicity induced by Fluoxetine. </span></span><br> &nbsp; Ashwagandha, adaptogen, infertility, selective serotonin reuptake inhibitors (SSRIs), fluoxetine, lipid peroxidation, oxidative stress, spermatogenesis 105 112 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1349-1&slc_lang=en&sid=1 Amira Siddiq chemistmera@gmail.com 100319475328460018994 0009-0007-5680-8411 No Zoology Department, Faculty of Science, Al-Azhar University, Assiut Branch, Egypt. Alaa M. H. El-Bitar elbitar@azhar.edu.eg 100319475328460018995 100319475328460018995 No Zoology Department, Faculty of Science, Al-Azhar University, Assiut Branch, Egypt. Abdelbaset M. A. Abdelreheem baset_m2002@azhar.edu.eg 100319475328460018996 100319475328460018996 No Zoology Department, Faculty of Science, Al-Azhar University, Assiut Branch, Egypt. Mohammed I Elkhateeb m_taha@azhar.edu.eg 100319475328460018997 0009-0009-0679-719X Yes Zoology Department, Faculty of Science, Al-Azhar University, Assiut Branch, Egypt.