en عمومى General پژوهشي Research <span style="font-size:12px;"><span style="font-family:Tahoma;"><b><span style="color:#2d73b4">Background: </span></b>Hepatotoxicity is liver damage caused by exposure to xenobiotics, resulting when the liver is unable to detoxify free radicals, such as reactive oxygen species (ROS), or other harmful metabolites. <i>Moringa oleifera</i> has been reported to possess preventive and therapeutic potential against various diseases due to its extract and bioactive components, particularly quercetin, which plays a significant role in its hepatoprotective effects. The present study aimed to evaluate the hepatoprotective potential of ethanolic <i>Moringa oleifera </i>leaf extract in carbon tetrachloride (CCl₄)-induced rats.<br> <b><span style="color:#2d73b4">Methods: </span></b>A total of 25 male Wistar rats were divided into five groups: a negative control group (0.9% NaCl), a positive control group (CCl₄), and three treatment groups receiving <i>Moringa oleifera</i> extract at doses of 250 mg/kg body weight (D1), 500 mg/kg body weight (D2), and 1000 mg/kg body weight (D3), all previously induced with 10 % CCl₄ solution administered at 1 mL/kg body weight. Observations were made on aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, as well as relative liver weight.<br> <b><span style="color:#2d73b4">Results: </span></b>Administration of ethanolic <i>Moringa oleifera</i> leaf extract at all three doses significantly reduced AST and ALT levels compared to the CCl₄ group, indicating hepatoprotective effects. However, no significant differences in relative liver weight were observed among the treatment groups.<br> <b><span style="color:#2d73b4"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b>Ethanolic <i>Moringa oleifera </i>leaf extract exhibits hepatoprotective effects against CCl₄-induced liver injury through the reduction of AST and ALT levels by 46.5% and 37%, respectively, although it does not influence relative liver weight.</span></span><br> &nbsp; Alanine aminotransferase (ALT), Aminotransferase (AST), hepatoprotective, Moringa oleifera, Relative liver weight. 71 76 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1437-1&slc_lang=en&sid=1 Annisa Dwi Lestari dlestariannisa@gmail.com 100319475328460018927 0009-0006-7760-3100 No Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Indonesia. R. Susanti basanatha@mail.unnes.ac.id 100319475328460018928 100319475328460018928 Yes Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Indonesia. Ari Yuniastuti ariyuniastuti@mail.unnes.ac.id 100319475328460018929 100319475328460018929 No Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Semarang, Semarang, Indonesia.