en عمومى General پژوهشي Research <span style="font-size:12px;"><span style="font-family:Tahoma;"><b><span style="color:#2d73b4">Background: </span></b>The present study was carried out to identify potential effects of dexamethasone (DEX) on antioxidant parameters of <i>Acanthopagrus arabicus</i>, <i>in vitro</i>.<br> <b><span style="color:#2d73b4">Methods: </span></b>Cultured hepatocytes from <i>A. arabicus</i> were exposed to 3, 3&times;10, 3&times;10&sup2;, and 3&times;10&sup3; &micro;g/ml of DEX for 48 h. Then, the toxic effects of DEX on the total antioxidant capacity (TAC), glutathione (GSH) enzyme activity, lipid peroxidation (LPO), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were measured at 12, 24, and 48 h of the experiment.<br> <b><span style="color:#2d73b4">Results: </span></b>The results indicated that the sensitivity of cultivated liver cells to DEX increased in a dose-dependent manner. The levels of TAC and GSH activity increased significantly in cells exposed to 3 and 3&times;10 &micro;g/ml of DEX after 48 h of the experiment. In contrast, higher concentrations of DEX (3&times;10&sup2; and 3&times;10&sup3; &micro;g/ml) induced a significant decrease in TAC value and GSH activity in treated hepatocytes throughout the experiment. The LPO, AST, ALT, and ALP activities increased dose- and time-dependently in cultivated hepatocytes exposed to 3&times;10&sup2; and 3&times;10&sup3; &micro;g/ml and reached their peak following 48 h of exposure.<br> <b><span style="color:#2d73b4"><span style="letter-spacing:-.1pt">Conclusion: </span></span></b>DEX can suppress the antioxidant defense system, as evidenced by the significant increase in lipid peroxides and decrease in antioxidant factors in the liver.<br> <span lang="EN" style="font-size:8.0pt"></span></span></span> Acanthopagrus arabicus, Dexamethasone, Glutathione, Liver enzymes, Total antioxidant capacity 130 138 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-1476-1&slc_lang=en&sid=1 ahmad Gabari ahmad.J@gmail.com 100319475328460019597 100319475328460019597 No Department of Marine Biology, Faculty of Marine Science and oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. Negin Salamat salamatnegin@yahoo.com 100319475328460019598 100319475328460019598 Yes Department of Marine Biology, Faculty of Marine Science and oceanography, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran. Asma Mohammadi as.mohamadi@yahoo.com 100319475328460019599 100319475328460019599 No Department of Basic Sciences, Abadan University of Medical Sciences, Abadan, Iran.