en The Effect of Rebadioside A on Attenuation of Oxidative Stress in ‎Kidney of Mice under Acetaminophen Toxicity تخصصي Special پژوهشي Research <b>Background: </b>Acetaminophen (APAP) overdose causes renal and hepatic injury. It is ‎also believed that oxidative stress has a pivotal role in APAP-induced renal injury. ‎Therefore, protective effects of different antioxidants have been examined in APAP-‎induced renal and hepatic toxicity models. Stevia rebadiana is a plant with a high ‎degree of natural antioxidant activity in its leaf extract. The aim of this study was to ‎investigate the possible protective effects of rebadioside A one of the main components ‎of stevia extract, on APAP-induced oxidative stress in kidney of mice.<br><b> ‎ Methods:</b> Oxidative stress was induced in kidney of BALB/c mice by the intraperitoneal ‎‎(i.p.) administration of a single dose of 300 mg/kg APAP. Some of these mice also ‎received rebadioside A (700 mg/kg) (i.p.) 30 minutes after APAP injection. Two and six ‎hours after APAP injection, all mice were sacrificed and malondialdehyde (MDA), ‎glutathione (GSH), free APAP, and glutathione conjugated of APAP (APAP-GSH) were ‎determined in the kidney tissue.‎<br> <b>Results:</b> GSH depletion and MDA levels significantly (P<0.05) increased in mice treated ‎with either APAP or APAP plus Rebadioside A, respectively in 2 and 6 hours intervals ‎after APAP administration. Significantly (P<0.05) higher levels of free APAP and ‎APAP-GSH levels detected in kidney of mice administrated with APAP plus rebadioside ‎A compared to APAP treated ones.‎ <br><b>Conclusion:</b> Rebadioside A may be a potential compound in alleviation of APAP-‎induced oxidative stress in kidney of mice after APAP overdoses. ‎ Acetaminophen, Oxidative Stress, Rebadioside A.‎ Acetaminophen, Oxidative Stress, Rebadioside A.‎ 944 951 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-2-138&slc_lang=en&sid=1 Seyed Ali Hashemi سیدعلی هاشمی 10031947532846002285 10031947532846002285 No Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran.‎ Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran.‎ Abdolamir ‎ Allameh عبدالامیر علامه allameha@modares.ac.ir 10031947532846002286 10031947532846002286 Yes Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran.‎ Department of Clinical Biochemistry, Tarbiat Modares University, Tehran, Iran.‎ Bahram Daraei بهرام دارایی 10031947532846002287 10031947532846002287 No Department of Toxicology, Tarbiat Modares University, Tehran, Iran.‎ Department of Toxicology, Tarbiat Modares University, Tehran, Iran.‎ Kamran Moradi Peynevandi کامران مرادی پینوندی 10031947532846002288 10031947532846002288 No ‎ Department of Plant Physiology, Tarbiat Modares University, Tehran, Iran.‎ ‎ Department of Plant Physiology, Tarbiat Modares University, Tehran, Iran.‎ Ramin Pashazadeh‎ رامین پاشازاده 10031947532846002289 10031947532846002289 No Department of Chemistry, Tarbiat Modares University, Tehran, Iran.‎ Department of Chemistry, Tarbiat Modares University, Tehran, Iran.‎