en Effects of Beta-Naphthoflavone and 3-Methylcholantheren on Biochemical Markers in Sturgeon Fish, Huso huso عمومى General پژوهشي Research <p dir="ltr"><strong>Background:</strong> Polycyclic aromatic hydrocarbons (PAHs) are a predominant group of contaminants that have been shown to accumulate and persist in marine organisms. We evaluated the dose-dependent induction of the cytochrome P4501A (CYP1A) system in the Sturgeon fish, <em>Huso huso </em>after after exposure to PAHs, beta-naphthoflavone (BNF) and 3- methylcholantheren.</p> <p dir="ltr"><strong>Methods:</strong> The fish were treated by i.p. injections of <a name="OLE_LINK7"></a><a name="OLE_LINK6">beta&ndash;naphthoflavone and 3- methylcholantheren </a>dissolved in corn oil at three doses 35, 70 and 105 mg/kg wet-body weight respectively for 72 h every day. Similarly, the control fish received injections of only corn oil. Microsomal fraction was prepared and ethoxy resorufin-O-deethylase activity (EROD) was measured in the fish liver. Cytochrome P4501A1 (CYP1A1) content was estimated by ELISA based on monoclonal anti-cod P4501A1.</p> <p dir="ltr"><strong>Results:</strong> Beta-naphthoflovone and 3-methylcholantheren treated-fish developed 15-32 fold increase in Ethoxyresorufin-O-deethylase activity in liver microsomes. The results of ELISA showed higher levels of cytochrome P4501A content in treated fish as compared to controls.</p> <p dir="ltr"><strong>Conclusion:</strong> A parallel increase of the CYP1A immunochemically assay and an increase in EROD activity could be recorded for beta &ndash;naphthoflavone and 3- methylcholantheren in injected fish. Dose-response relationships on the induction of EROD and CYP1A immunodetectable protein were recorded. Therefore, EROD activity and CYP1A content can be used as a biomarker of polycyclic aromatic hydrocarbons.</p> 3-Methylcolantheren, Beta–Naphthoflavon, Biomarker, Ethoxyresorufin Deethylase, Huso Huso. 3-Methylcolantheren, Beta–Naphthoflavon, Biomarker, Ethoxyresorufin Deethylase, Huso Huso. 13 17 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-270-2&slc_lang=en&sid=1 katayoon karimzadeh کتایون کریم زاده karimzadehkatayoon@liau.ac.ir 10031947532846005177 10031947532846005177 Yes Department of Marine Biology, Islamic Azad University, Branch of Lahijan 1616, Lahijan, Iran. Department of Marine Biology, Islamic Azad University, Branch of Lahijan 1616, Lahijan, Iran.