en Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils تخصصي Special پژوهشي Research <p dir="ltr"><strong>Background:</strong> Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran.</p> <p dir="ltr"><strong>Methods:</strong> Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent<a name="m_-56562732264333694_OLE_LINK1"></a><a name="m_-56562732264333694_OLE_LINK2"></a> degrading strains was performed using PCR based on amplifying of 16s rDNA.</p> <p dir="ltr"><strong>Results:</strong> The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environment. <a name="m_-56562732264333694_OLE_LINK5"></a><a name="m_-56562732264333694_OLE_LINK6"></a>Three diazinon-degrading bacteria strains belong to: <em>Pseudomonas putida </em>strain D<em>3,</em> <em>P. fluorescens strain </em>D1 and<em> Achromobacter piechaudii </em>strain D8. The best degrading strain (D1), up to 100 ppm, illustrated a good growth, whereas more than this concentration, the growth was reduced. The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.</p> <p dir="ltr"><strong>Conclusion:</strong> The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.</p> <p dir="ltr">Using these strains and other biological reclamation methods we can eliminate bio-environmental problems.</p> Agricultural Pesticide, Degrading Bacteria, Diazinon 13 20 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-357-1&slc_lang=en&sid=1 Mehdi Hassanshahian مهدی حسن شاهیان mshahi@uk.ac.ir 100319475328460011180 100319475328460011180 Yes Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran. Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran.