en عمومى General پژوهشي Research <p>Background: Cutaneous reactions like pruritus and erythema are common in warm months of the year in Bushehr Port, Persian Gulf, Iran due to jellyfish envenomation. This study reports isolation of the Chrysaora hysoscella nematocysts and evaluating its pharmacological activities during a bloom in 2013.</p> <p>Methods: The venom of C. hysoscella captured in Jofre area in Bushehr port was analyzed. The electrophoretic profile was assessed by SDS-PAGE (12.5%) and the crude sample was analyzed using reverse phase HPLC. Caseinase activity was also determined.</p> <p>Results: After separation of tentacles and isolation of their nematocysts, three different major protein components were revealed at 72-250 kDa with SDS-PAGE, signifying the presence of peptides in its venom. Two major peaks at 8.62 and 11.23 min were observed in reverse phase HPLC of the crude venom denoting protease peptide structural identities. <a name="OLE_LINK2"></a><a name="OLE_LINK1">Caseinase </a>activity of C. hysoscella&#39;s venom was extremely low as compared with other jellyfish venoms.</p> <p>Conclusion: This was the first report on the structural examination of jellyfish in Persian Gulf and may pave the way for determination and separation of destructive enzymes inducing cutaneous reactions in fishermen and swimmers.</p> Caseinase, Cnidarian Venoms, Nematocyst, Scyphozoa, Sea Nettle. 7 10 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-76-1&slc_lang=en&sid=1 Somayyeh Gharibi gharibi.somayyeh@gmail.com 10031947532846005501 10031947532846005501 No Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran. Iraj Nabipour nabipourpg@bpums.ac.ir 10031947532846005502 10031947532846005502 No Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran. Euikyung Kim ekim@gnu.ac.kr 10031947532846005503 10031947532846005503 No PhD of Toxicology, College of Veterinary Medicine, Gyeoungsang National University, Jinju, South Korea. Seyede Maryam Ghafari ghafarykm@gmail.com 10031947532846005504 10031947532846005504 No Department of Parasitology, Pasteur Institute, Tehran, Iran. Seyed Mehdi Hoseiny mehdi_h117@yahoo.com 10031947532846005505 10031947532846005505 No Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Science Mostafa Kamyab mostafa.kamyab@gmail.com 10031947532846005506 10031947532846005506 No Department of Biological Sciences, Shahid Beheshti University, Tehran, Iran. Ramin Seyedian raminseyedian@gmail.com 10031947532846005507 10031947532846005507 Yes Department of Marine Toxicology, The Persian Gulf Marine Biotechnology Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.