en عمومى General پژوهشي Research <strong>Background:</strong> The global incidence of snakebite has become a major concern to the community. This study aimed to evaluate the effect of mango seed kernel methanol extract on metalloproteases in Carpet Viper (<em>Echis ocellatus</em>) venom.<br> <strong>Methods:</strong> Mango seed kernel methanolic extract was evaluated in vitro for its anti-venom activity and inhibition of metalloproteases of Carpet Viper&#39;s (<em>Echis ocellatus</em>) venom. Metalloprotease portion was partially purified from the venom of <em>E. ocellatus </em>with a yield of 71%, a purification fold of 2.63 and a specific activity of 19.00 &micro;mol/min/mg protein.<br> <strong>Results:</strong> The enzyme appeared as a band on SDS-PAGE with a molecular weight of 23 kDa. The kinetic properties of the enzyme showed a K_m of 0.31 mg mL^-1and a V_max of 9.09 &micro;mol min^-1. When the enzyme was incubated with the extract, kinetic studies revealed a mixed non-competitive pattern of inhibition with K_m values of 0.56 and 1.11 mg mL^-1and V_maxvalues of 6.67 and 4.17-&micro;mol min^-1for 5% and 20% inhibitor concentrations, respectively. An estimated K<em>_i</em> value of 0.168 mg mL^-1was obtained from a secondary plot demonstrating that the extract had a high affinity for the partially purified enzyme; thus, could serve as an effective inhibitor.<br> <strong>Conclusion:</strong> Methanol extract of mango seed kernel has a high affinity for the partially purified enzyme, and it might provide an inexpensive and readily available alternative to sheep serum in the management of snakebite envenomation. Therefore, further in vivo studies are necessary to assess its effectiveness and safety. Mangifera indica, Snake venom, Metalloprotease, Inhibition binding constant Mangifera, Metalloproteases, Snake Bites, Viperidae, Viper Venoms 23 30 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-416-1&slc_lang=en&sid=1 Peculiar Nwanyibunwa Okoro peckyclett@sironigeria.com 10031947532846006472 10031947532846006472 Yes Department of Basic Research, National Research Institute for Chemical Technology, Zaria, Nigeria. Sani Ibrahim 10031947532846006473 10031947532846006473 No Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria. Hajiya Mairo Inuwa 10031947532846006474 10031947532846006474 No Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria. Stanley Irobekhian Reuben Okoduwa 10031947532846006475 10031947532846006475 No Department of Research and Development, Nigerian Institute of Leather and Science Technology, Zaria, Nigeria.