en تخصصي Special پژوهشي Research <p><strong>Background:</strong> Phthalates are widely used as plasticizers in polyethylene terephthalate (PET) products. Several pharmaceutical products have been stored in PET containers, and due to serious health effects of phthalates, migration levels of them into pharmaceutical products must be determined. In the present study, leaching levels of four common phthalate esters including di-n-butyl phthalate (DBP), diethyl phthalate (DEP), benzyl butyl phthalate (BBP) and Di-(2-ethylhexyl) phthalate (DEHP) into various types of infusion fluids form four different commercial brand were investigated.</p> <p><strong>Methods:</strong> Trace levels of phthalate esters were successfully extracted by a dispersive liquid-liquid microextraction (DLLME) method using acetonitrile as dispersive and CCL₄ as an extraction solvent and analyzed by gas chromatography-mass spectrometry (GC-MS).</p> <p><strong>Results:</strong> Different levels DEHP were detected in all samples (mean=10.55&plusmn;6.88 and maximum=19.7 ppb). While no levels of other phthalates were detected in some samples, some other contained up to 25.1&plusmn;17.7, 15.4&plusmn;8.3, 8.1 ppb DEP, DBP, and BBP respectively. Mean total phthalate ester (TPE) levels in infusion fluids were 7.92&plusmn;5.68 ppb. Phthalate leaching into normal saline was significantly lower than other types of serums and brand ID#3&rsquo; products contain minimum phthalate levels compared to other brands.</p> <p><strong>Conclusions:</strong> Hospitalized patient particularly those who regularly take serum-therapy and children are at significant risk of phthalate exposure via intravenous fluids receiving.</p> Exposure Assessment, Hospitalized Patients, Intravenous Fluids, Phthalate Esters, Toxicity 33 38 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-425-1&slc_lang=en&sid=1 Forouz Rastegari 10031947532846006399 10031947532846006399 No MSc of Chemical Toxicology, Environment Research Center, Research Institute for Primordial Prevention of Non-communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran. Mohammad Mehdi Amin 10031947532846006400 10031947532846006400 No Department of Environmental Health Engineering, Isfahan University of Medical Sciences, Isfahan, Iran. Karim Ebrahim k.najafabady@sbmu.ac.ir 10031947532846006401 10031947532846006401 Yes Department of Environmental Health Engineering, Isfahan University of Medical Sciences, Isfahan, Iran.