en عمومى General پژوهشي Research <strong>Background:</strong> This study intended to assess individual and combined adsorption potentials of three adsorbents (processed bentonite as an inorganic adsorbent, and cell walls of <em>Saccharomyces cerevisiae</em> and of the GG strain of <em>Lactobacillus rhamnosus</em> as organic adsorbents) for aflatoxin B₁ and ochratoxin A under in vitro conditions.<br> <strong>Methods:</strong> This study was conducted in Ferdowsi University of Mashhad, Mashhad, Iran in 2106. A pre-test with four treatments of bacteria and yeast (live or dead) with five replications was designed and conducted to study the effects of these biotic and abiotic organic adsorbents on toxin adsorption efficiency. The experiment in the main study had seven treatments including two toxins (2 ppm) and three adsorbents with five replications using the completely randomized design. Toxin quantities were measured by an HPLC instrument.<br> <strong>Results:</strong> The various types of dead organic adsorbents were more capable of adsorbing toxins compared to the live ones. The processed bentonite was considerably more efficient in adsorbing aflatoxin B₁ (93.51) compared to the other treatment groups (<em>P</em><0.05). No significant differences were observed between the effects of yeast and bacterial cell walls in adsorbing aflatoxin B₁. However, the bacterial cell walls (61.71) had higher adsorption efficiencies in adsorbing ochratoxin A than the processed bentonite and yeast cell walls (<em>P</em><0.05).<br> <strong>Conclusion:</strong> Processed bentonite (or montmorillonite) and bacterial cell walls are able to adsorb considerable quantities of aflatoxin B₁and ochratoxin A, and can be used in multiple mycotoxin contaminations as an effective strategy for preventing or reducing the harmful effects of these toxins. Adsorbents, Bentonite, Detoxification, Lactobacillus, Mycotoxin, Yeast Cell Wall 7 13 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-486-1&slc_lang=en&sid=1 Damoon Ghofrani Tabari 10031947532846007261 10031947532846007261 No Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. Hassan Kermanshahi hassbird@yahoo.com 10031947532846007262 10031947532846007262 Yes Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. Abolghasem Golian 10031947532846007263 10031947532846007263 No Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran. Reza Majidzadeh Heravi 10031947532846007264 10031947532846007264 No Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.