en عمومى General پژوهشي Research <strong>Background:</strong> <em>Aspergillus flavus</em> is the most important fungus for production of Aflatoxin B1 (AFB1). This study evaluated the ability of gamma rays (GRs) and electron-beam irradiation (EBI) to counteract the deleterious effects of aflatoxin B1 (AFB1) in a chicken model.<br> <strong>Methods:</strong> Overall, 168 one-day-old male Coturnix quails were assigned to eight treatments for 42 d in Tehran, Iran, in 2010 and 2011. Two dietary inclusion rates of AFB1 (0 and 2 ppm) and toxin binders, such as 0, 27 kGy doses of GRs, 27 kGy doses of EBI, and 0.3% of commercial toxin binder-milbond-TX, were tested in a 2&times;4 factorial manner. Serum biochemical parameters, immune response, and dietary treatments on factors associated with kidney and lipid profiles were determined on day 42.<br> <strong>Results:</strong> AFB1 significantly decreased the hematological parameters (Hematocrit in 21 and 42 d), immune response (White blood cell (WBC), heterophil to lymphocyte ratio (H/L) and sheep red blood cell (SRBC)), and blood chemical factors (glucose, albumin, total protein, and triglycerides) compared to the control diet (<em>P</em><0.05). It also significantly increased the calcium, phosphorus, uric acid, and low-density lipoprotein (LDL) levels (<em>P</em><0.05). The addition of toxin binders, such as GRs, EBI, and milbond-TX, in the contaminated diets significantly diminished the inhibitory effects of dietary AFB1 (<em>P</em><0.05) on the hematological parameters, immune response, blood chemical factors, and factors associated with kidney and lipids profile with no differences compared to the control diet.<br> <strong>Conclusion:</strong> The addition of these toxin binders may reduce the adverse effects produced by the presence of AFB1 in Japanese quails&rsquo; diets. Aflatoxin B1, Electron-Beam Irradiation, Gamma Rays, Japanese Quail, Milbond-TX 15 20 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-65-3&slc_lang=en&sid=1 Saeed Hasanpour 10031947532846007281 10031947532846007281 No Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. Shaban Rahimi 10031947532846007282 10031947532846007282 No Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran. Omid Fani Makki ofanimakki@birjand.ac.ir 10031947532846007283 10031947532846007283 Yes MSc of Poultry Science, Research and Development Office, Zarin Gostar Sarina Company, Kashmar, Iran. Gholamreza Shahhosseini 10031947532846007284 10031947532846007284 No Department of Agricultural Research, Medicine and Industrial Research School, Karaj, Iran. Alireza Khosravi 10031947532846007285 10031947532846007285 No Department of Mycology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.