en تخصصي Special پژوهشي Research <strong>Background:</strong> The therapeutic potential of honey is related to antioxidant activity against reactive oxygen species because it contains compounds such as polyphenols; therefore, we evaluated the potential protective effect of honey on subacute toxicity of ACR by histopathologic study on tissue lesions in rat.<br> <strong>Methods:</strong> In Ferdowsi University of Mashhad, Mashhad, Iran, 2016, male Wistar rats were divided into 7 groups<strong>.</strong> To induce toxicity, ACR was injected (50 mg/kg for 11 d) to rats in 5 groups. In treatment groups, rats received three doses of honey 1.25, 2.5, and 5 g/kg in addition to the ACR. The two remaining groups received vitamin E (200 IU/kg) and normal saline as positive and negative control respectively. On the last day, after necropsy, tissue specimens from brain and liver were collected for histopathological studies.<br> <strong>Results:</strong> Receiving of ACR caused tissue injuries including degeneration, necrosis, hyperemia, hemorrhage and inflammation in liver; ischemic cell change, hyperemia, hemorrhage and edema in brain tissue. Administration of honey considerably reduced tissue damages caused by ACR, particularly with dosage 5 g/kg.<br> <strong>Conclusion:</strong> The severity of tissue lesions caused by the ACR can be reduced by honey, likely through its antioxidant activity. Increasing concentrations of honey will enhance its effectiveness. Acrylamide, Histopathology, Honey, Wistar Rat 1 8 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-511-1&slc_lang=en&sid=1 Parichehr Ahrari Roodi 100319475328460011204 0000000323731984 No DVM, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Zahra Moosavi zmoosavi@um.ac.ir 100319475328460011205 0000000283882699 Yes Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Amir Afkhami Goli 100319475328460011206 100319475328460011206 No Department of Basic Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Mohammad Azizzadeh 100319475328460011207 100319475328460011207 No Department of Clinical Science, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran. Hossein Hosseinzadeh 100319475328460011208 100319475328460011208 No Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.