Iranian Journal of Toxicology
مجله سم شناسی و مسمومیتهای ایران
IJT
Medical Sciences
http://ijt.arakmu.ac.ir
1
admin
2008-2967
2251-9459
8
10.61186/ijt
14
8888
13
en
jalali
1397
4
1
gregorian
2018
7
1
12
4
online
1
fulltext
en
Cytotoxic Activity of Familact: A Probiotic Supplement
عمومى
General
پژوهشي
Research
<strong>Background:</strong> <em>Lactobacillus</em> and <em>Bifidobacterium</em> species are among the probiotics discussed due to their anti-cancer effects in the treatment of colorectal and breast cancers in recent studies. The aim of this study was to investigate the anticancer effect of Familact, a commercial probiotic capsule containing seven bacterial strains (<em>L. casei</em>, <em>L. acidophilus</em>, <em>L. rhamnosus, L. bulgaricus,</em> <em>B. breve,</em> <em>B. longum</em> and <em>Streptococcus thermophilus</em>).<br>
<strong>Methods:</strong> Various cancer cell lines including Caco-2, HT-29, T47D and normal cell line L929 were treated with different concentrations of Familact. Using MTT assay, the cytotoxicity effect was investigated for each cell line and then flow cytometry analysis of apoptosis was evaluated.<br>
<strong>Results:</strong> Familact demonstrated inhibitory effects on the proliferation of all tested cancer cell lines in a dose-dependent manner. Although Familact augmented apoptotic cell death in HT-29 human cancer cells, it was less effective in the case of Caco-2 and T47D cells. Moreover, exposure to Familact showed moderate cytotoxicity towards L929 mouse fibroblast cells.<br>
<strong>Conclusion:</strong> Familact could be considered as a complementary therapy in the treatment of cancers.
Apoptosis, Breast Cancer, Colorectal Cancer, Familact, Prebiotic, Probiotics
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http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-533-1&slc_lang=en&sid=1
Zahra
Yahyavi
100319475328460011275
100319475328460011275
No
Department of Microbiology, Faculty of Basic Sciences, Karaj Branch, Islamic Azad University, Alborz, Iran.
Mohammad Reza
Fazeli
100319475328460011276
100319475328460011276
No
Department of Drug & Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Mani
Mirfeizi
100319475328460011277
100319475328460011277
No
Department of Midwifery, College of Nursing and Midwifery, Karaj Branch, Islamic Azad University, Karaj, Iran.
Shima
Aliebrahimi
100319475328460011278
100319475328460011278
No
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Seyed Nasser
Ostad
ostadnas@tums.ac.ir
100319475328460011279
100319475328460011279
Yes
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.