en عمومى General پژوهشي Research <strong>Background</strong>:&nbsp;<em>Maytenus heterophylla (M. heterophylla) </em>is commonly used in African traditional medicine for the management of various ailments. The present study evaluated the antioxidant, antimicrobial and safety properties of the Flavonoid extract of <em>M. heterophylla </em>in Wister rats.<br> <strong>Methods</strong>: The Flavonoid was subjected to antibacterial study via agar well diffusion method, and antioxidant study using 2, 2&prime;-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant properties (FRAP) assays. Subacute toxicity were carried out by the oral administration of the extract at a daily dose of 50 or 100mg/kg for 28 days.<br> <strong>Results</strong>:&nbsp;The extract produced significant antioxidants activities with IC₅₀ of 33.07&plusmn;0.84 &micro;g/mL & 38.08&plusmn;0.89 &mu;g/mL in DPPH and FRAPS models respectively. It produced a dose-dependent inhibition of <em>S. aureus, E.coli, P. aeruginosa, K. pneumonia</em> and <em>S. Typhi </em>with MIC between 12.5&micro;g/mL to 25&micro;g/mL. The flavonoid was safe on acute exposure to rats (LD₅₀> 5000 mg/kg). However, the&nbsp; chronic exposure significantly (p<0.05) decreased the creatinine, bilirubin concentrations and increased&nbsp; aspartate transaminase (AST) activities while the total protein, albumin, alanine transaminase (ALT), alkaline phosphatise (ALP), urea, chloride, potassium and sodium concentrations were comparable with those in the controls. The organs-body weights ratios also compared well with the controls (p<0.05).<br> <strong>Conclusions</strong>: The findings showed that the Flavonoid extract of <em>M. heterophylla </em>was relatively non-toxic following acute or chronic exposures at 50-100 mg/kg. The flavonoid extract may potentially serve as a candidate agent for the development of an anti-microbial drug and to enhance the antioxidant capacity in rats.<br> &nbsp; Antibacterial, Anti-Oxidants, Flavonoids, Maytenus Heterophylla, Toxicity 39 44 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-516-2&slc_lang=en&sid=1 Sheriff Itopa Umar 100319475328460011502 100319475328460011502 No MTech of Biochemistry, Federal Polytechnic, Ile-Oluji, Ondo State, Nigeria. Bashir Lawal bashirlawal12@gmail.com 100319475328460011503 100319475328460011503 Yes Department of Biochemistry, Federal University of Technology, Minna, Nigeria. Bala Alkali Mohammed 100319475328460011504 100319475328460011504 No Department of Biochemistry, Federal University of Technology, Minna, Nigeria. Cyril Ifeanyi Obiekezie 100319475328460011505 100319475328460011505 No Department of Biochemistry, Federal University of Technology, Minna, Nigeria. Abdulsalam Hassan Adewuyi 100319475328460011506 100319475328460011506 No Department of Biochemistry, Federal University of Technology, Minna, Nigeria. Shukurat Bisola Babalola 100319475328460011507 100319475328460011507 No Department of Chemistry, Federal University of Technology, Minna, Nigeria. Stephen Damaola Ariyeloye 100319475328460011508 100319475328460011508 No Department of Biochemistry, Federal University of Technology, Akure, Nigeria.