en عمومى General پژوهشي Research <strong>Background</strong>: Acetaminophen-induced toxicity is a common cause of acute liver failure. This study investigated the hepatoprotective effects of <em>Eryngium bungei B</em>oiss (EB) extract against the hepatotoxicity in rats.<br> <strong>Methods</strong>: Thirty adult male rats were randomly assigned to five groups, with free access to water and food. They were treated as follows: <em>Group 1</em> (negative controls): distilled water (DW); <em>Group 2</em> (positive controls): DW for 7 days and a single dose of 500 mg/kg BW acetaminophen orally on day 8^th; <em>Groups 3, 4 </em>and<em> 5</em> (experimental groups): received EB extract mixed in DW at 100, 200 and 400 mg/kg/day orally for 7 days plus 500 mg/kg acetaminophen on the 8^th day. On the 9thday, blood and liver samples were collected from all rats.<br> <strong>Results</strong>: The EB extract improved the adverse histological changes in the rats&rsquo; livers and resulted in reduced serum ALT and ALP enzymes. Oxidative stress was noted in the liver tissue in <em>Group 2</em>. Pretreatment with EB extract reduced MDA concentration and increased GSH levels, compared to that for <em>Group 2</em>. The extract at 200 and 400 mg/kg/day significantly increased SOD activity, compared to that for <em>Group 2</em>. The IL-1&beta; and TNF-&alpha; levels increased significantly in <em>Group 2</em>, compared to those in <em>Group 1</em>. Administration of EB extract in Groups 3, 4 and 5 significantly decreased the IL-1&beta; and TNF-&alpha; parameters.<br> <strong>Conclusions</strong>: The hepatoprotective effects of EB extract appears to be linked to its glutathione-mediated detoxification, free radical scavenging and anti-inflammatory properties. Acetaminophen Hepatotoxicity, Eryngium Bungei Extract, Inflammation, Oxidative Stress, Rats 11 16 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-604-1&slc_lang=en&sid=1 Maryam Fatemi 100319475328460011515 100319475328460011515 No Department of Basic Sciences, School of Veterinary Medicine, Shiraz University. Shiraz, Iran. Tahoora Shomali tshomali@shirazu.ac.ir 100319475328460011516 100319475328460011516 Yes Department of Basic Sciences, School of Veterinary Medicine, Shiraz University. Shiraz, Iran. Saeed Nazifi 100319475328460011517 100319475328460011517 No Department of Clinical Studies, School of Veterinary Medicine, Shiraz University. Shiraz, Iran. Mehdi Fazeli 100319475328460011518 100319475328460011518 No Department of Basic Sciences, School of Veterinary Medicine, Shiraz University. Shiraz, Iran.