en عمومى General پژوهشي Research <div style="text-align: justify;"><strong>Background</strong>: Chromolaena odorata (C. odorata) is a medicinal plant with hypoglycemic effect known in traditional medicine. The present study investigated the in vitro antioxidant and in vivo anti-hyperglycemic effect of the methanol root extract of C. odorata in rats.<br> <strong>Methods</strong>: Phytochemical compositions were evaluated, using standard procedures while the antioxidant activities were examined, using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging and ferric reducing power (FRAP) assays. Twenty albino rats were administered 120 mg/kg of body weight (bw) alloxan and were divided into four groups of five animals each. Groups A to D were treated with 2 ml/kg of normal saline, 300 mg/kg, 500 mg/kg bw extract and 5 mg/kg bw glibenclamide, respectively, through oral route for 14 days. Blood samples were collected and prepared for the analysis of serum lipids profile.<br> <strong>Results</strong>: The extract contained tannins (54.76&plusmn;4.08 mg/100 g), saponins (322.78&plusmn;17.35 mg/100 g), glycosides, alkaloids (74.34&plusmn;6.08 mg/100 g), flavonoids (79.63&plusmn;4.55 mg/100 g), phenols (154.32&plusmn;11.21 mg/100 g), glycosides, steroids and reducing sugar. The extract promoted the inhibition of DPPH radicals with IC50 values of 191.68&plusmn;1.68 &micro;g/mL. In comparison with the untreated controls, total cholesterol, Low Density Lipoprotein (LDL)-cholesterol and triglycerides were significantly reduced (P<0.05) following treatment with C. odorata extract while high density lipoprotein (HDL)-cholesterol, was significantly increased.&nbsp;<br> <strong>Conclusion</strong>: The C. odorata extract exhibited antioxidant and hypolipidemic effects in alloxan-induced diabetic rat, thus could be considered as a natural product in the management of dyslipidemia secondary to diabetes.<br> &nbsp;</div> Antioxidants, Chromolaena odorata, Diabetes mellitus, Hypolipidemic, Lipid profile Antioxidants, C. odorata extract, Diabetes mellitus, Hypolipidemia, Blood lipid profile 63 70 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-612-1&slc_lang=en&sid=1 Oluyemisi Omotayo Omonije oluyemisiomonije@gmail.com 10031947532846009450 10031947532846009450 Yes Department of Biochemistry, Federal University of Technology, Minna, Nigeria. Abubakar Ndaman Saidu 10031947532846009451 10031947532846009451 No Department of Biochemistry, Federal University of Technology, Minna, Nigeria. Hadiza Lami Muhammad 10031947532846009452 10031947532846009452 No Department of Biochemistry, Federal University of Technology, Minna, Nigeria.