en تخصصي Special پژوهشي Research <div style="text-align: justify;"><span style="color:#000080;"><strong>Background</strong></span>: Multiple Sclerosis (MS) is a chronic autoimmune disease due to the demyelination of axons in the central nervous system. Melatonin, as a hormone with potential anti-inflammatory effects, can reduce the MS symptoms by altering the levels of inflammatory mediators.<br> <span style="color:#000080;"><strong>Methods</strong></span>: In this study, 50 MS patients referred to the MS Society in Markazi Province were randomly selected. Each patient completed and signed a consent form and was referred to the MS Center at Vali-Asr Hospital in Arak, Iran. All patients who were routinely receiving interferon, were divided into two groups of 25 each. Group 1 (control) received placebo, and Group 2 (treatment) received one dose of melatonin (3mg) per day for 24 weeks. Patients&rsquo; recorded anthropometric data included height, weight, and age. Fasting blood samples were collected and the serum levels of INF-1&beta; and VitB12 determined. The blood samples were immediately centrifuged to separate sera, which were kept in a freezer at -80&deg; C. The serum levels of INF-1&beta; and Vitamin B12 were determined, using ELISA kits.<br> <span style="color:#000080;"><strong>Results</strong></span>: The data showed that there were significant differences in the serum levels of INF-1&beta; and Vitamin B12 between the control and treatment groups. The levels were significantly increased in the treatment group compared to those in the controls, suggesting that melatonin might have induced the changes.<br> <span style="color:#000080;"><strong>Conclusion</strong></span>: Based on the results, the application of melatonin might be a valuable strategy in the treatment of patients with MS.</div> Multiple Sclerosis, Melatonin, INF-1β and VitB12 Multiple Sclerosis, Melatonin, INF-1β and Vitamin B12, Anti-inflammatory effect, Autoimmune disease 19 24 http://ijt.arakmu.ac.ir/browse.php?a_code=A-10-619-1&slc_lang=en&sid=1 Masoomeh Yosefi-Fard معصومه یوسفی فرد 62yousefifard@gmail.com 10031947532846008707 10031947532846008707 Yes Department of Biology, College of Biological Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran. دانشگاه آزاد اسلامی واحد دامغان Gholamhassan Vaezi غلامحسن واعظی Gh.vaezi@yahoo.com 10031947532846008708 10031947532846008708 No Department of Biology, College of Biological Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran. دانشگاه آزاد اسلامی واحد علوم تحقیقات Ali Akbar Maleki-Rad علی اکبر ملکی راد malekirad1973@gmail.com 10031947532846008709 10031947532846008709 No Toxicology and Diseases Group, Pharmaceutical Science Research Center, Tehran University of Medical Sciences, Tehran, Iran. دانشکده زیست شناسی ، دانشگاه پیام نور تهران.ایران و مرکز تحقیقات علوم دارویی دانشگاه علوم پزشکی تهران Fardin Faraji فردین فرجی fardin.faraji@yahoo.com 10031947532846008710 10031947532846008710 No Department of Neurology, School of Medicine, Arak University of Medical Sciences, Arak, Iran. |دانشکده علوم اعصاب دانشگاه علوم پزشکی اراک Vida Hojati ویدا حجتی vida.hojati@gmail.com 10031947532846008711 10031947532846008711 No Department of Biology, College of Biological Sciences, Damghan Branch, Islamic Azad University, Damghan, Iran. دانشگاه آزاد اسلامی واحد دامغان